The Arabidopsis thaliana cysteine-rich receptor-like kinases CRK6 and CRK7 protect against apoplastic oxidative stress
The Arabidopsis thaliana cysteine-rich receptor-like kinases CRK6 and CRK7 protect against apoplastic oxidative stress
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DOI：
10.1016/j.bbrc.2014.02.013
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发表时间：
2014-03-07
影响因子：
3.1
通讯作者：
Wrzaczek, Michael
中科院分区：
文献类型：
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作者：
Idanheimo, Niina;Gauthier, Adrien;Wrzaczek, Michael
受体样激酶是植物许多不同过程的重要调节因子。尽管它们数量众多，但只有少数具有功能特征。拟南芥中最大的受体样激酶亚群之一是富含半胱氨酸的受体样激酶（CRKs）。CRKs之间的高序列相似性被认为是功能冗余的主要原因。CRK基因在背靠背重复序列中的基因组定位使得通过突变体分析对其进行表征变得不现实。表达谱分析将CRKs与活性氧（植物中重要的信号分子）联系起来。在这里，我们研究了两个crk， CRK6和CRK7的作用，并分析了它们在细胞外ROS信号传导中的作用。CRK6和CRK7是活性蛋白激酶，对二价阳离子有不同的偏好。我们的研究结果表明，CRK7参与介导细胞外而非叶绿体ROS产生的反应。(C) 2014爱思唯尔公司版权所有。
Receptor-like kinases are important regulators of many different processes in plants. Despite their large number only a few have been functionally characterized. One of the largest subgroups of receptor-like kinases in Arabidopsis is the cysteine-rich receptor like kinases (CRKs). High sequence similarity among the CRKs has been suggested as major cause for functional redundancy. The genomic localization of CRK genes in back-to-back repeats has made their characterization through mutant analysis unpractical. Expression profiling has linked the CRKs with reactive oxygen species, important signaling molecules in plants. Here we have investigated the role of two CRKs, CRK6 and CRK7, and analyzed their role in extracellular ROS signaling. CRK6 and CRK7 are active protein kinases with differential preference for divalent cations. Our results suggest that CRK7 is involved in mediating the responses to extracellular but not chloroplastic ROS production. (C) 2014 Elsevier Inc. All rights reserved.