Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase, sorbitol dehydrogenase and sorbitol kinase.
Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase, sorbitol dehydrogenase and sorbitol kinase.
复制标题
通过牛和人晶状体醛糖还原酶、山梨醇脱氢酶和山梨醇激酶形成山梨醇 6-磷酸。
DOI：
10.1016/0304-4165(82)90171-4
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Petrash,JM
中科院分区：
文献类型：
--
作者：
Srivastava,SK;Ansari,NH;Brown,JH;Petrash,JM
已证明牛和人透镜醛糖还原酶和山梨醇脱氢酶分别通过还原葡萄糖6-磷酸和果糖6-磷酸形成山梨醇6-磷酸。反应产物经Dowex-甲酸根柱色谱、气相色谱和质谱鉴定。6-磷酸山梨糖醇也可通过在ATP存在下由透镜山梨糖醇激酶磷酸化山梨糖醇而形成。
Formation of sorbitol 6-phosphate by bovine and human lens aldose reductase and sorbitol dehydrogenase by the reduction of glucose 6-phosphate and fructose 6-phosphate, respectively, has been demonstrated. The reaction product has been identified by Dowex-formate column chromatography, gas chromatography and mass spectrometry. Sorbitol 6-phosphate can also be formed by the phosphorylation of sorbitol by lens sorbitol kinase in the presence of ATP.