Preparation and antibacterial activity of Fe3O4@Ag nanoparticles
Preparation and antibacterial activity of Fe3O4@Ag nanoparticles
复制标题
Fe3O4@Ag纳米颗粒的制备及其抗菌活性
DOI：
10.1088/0957-4484/18/28/285604
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发表时间：
2007-07-18
期刊：
影响因子：
3.5
通讯作者：
Yang, Xiaohai
中科院分区：
文献类型：
--
作者：
Gong, Ping;Li, Huimin;Yang, Xiaohai
采用油包水型微乳液法在Fe 3 O 4纳米粒子表面还原硝酸银，制备了具有超顺磁性和抗菌性能的双功能Fe3O4@Ag纳米粒子。通过透射电子显微镜和动态光散射证实形成了尺寸为60 ± 20 nm的分散良好的纳米颗粒。X-射线衍射和紫外-可见光谱表明，Fe 3 O 4和银存在于同一颗粒中。用振动样品磁强计证实了Fe3O4@Ag纳米粒子的超顺磁性。采用最低抑菌浓度、流式细胞仪和抑菌率测定等方法评价其抗菌活性。结果表明，Fe3O4@Ag纳米粒子对大肠杆菌（革兰氏阴性菌）、表皮葡萄球菌（革兰氏阳性菌）和枯草芽孢杆菌（芽孢菌）具有良好的抗菌性能。此外，Fe3O4@Ag纳米颗粒可以通过使用磁场容易地从水中去除，以避免污染周围环境。回收的Fe3O4@Ag纳米粒子仍具有抗菌能力，可重复使用。
Bifunctional Fe3O4@Ag nanoparticles with both superparamagnetic and antibacterial properties were prepared by reducing silver nitrate on the surface of Fe3O4 nanoparticles using the water-in-oil microemulsion method. Formation of well-dispersed nanoparticles with sizes of 60 ± 20 nm was confirmed by transmission electron microscopy and dynamic light scattering. X-ray diffraction patterns and UV–visible spectroscopy indicated that both Fe3O4 and silver are present in the same particle. The superparamagnetism of Fe3O4@Ag nanoparticles was confirmed with a vibrating sample magnetometer. Their antibacterial activity was evaluated by means of minimum inhibitory concentration value, flow cytometry, and antibacterial rate assays. The results showed that Fe3O4@Ag nanoparticles presented good antibacterial performance against Escherichia coli (gram-negative bacteria), Staphylococcus epidermidis (gram-positive bacteria) and Bacillus subtilis (spore bacteria). Furthermore, Fe3O4@Ag nanoparticles can be easily removed from water by using a magnetic field to avoid contamination of surroundings. Reclaimed Fe3O4@Ag nanoparticles can still have antibacterial capability and can be reused.