DIFFERENTIAL EXPRESSION OF 3 GAP JUNCTION PROTEINS IN DEVELOPING AND MATURE BRAIN-TISSUES
DIFFERENTIAL EXPRESSION OF 3 GAP JUNCTION PROTEINS IN DEVELOPING AND MATURE BRAIN-TISSUES
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DOI：
10.1073/pnas.86.24.10148
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发表时间：
1989-12-01
影响因子：
11.1
通讯作者：
WILLECKE, K
中科院分区：
文献类型：
--
作者：
DERMIETZEL, R;TRAUB, O;WILLECKE, K
通过使用针对肝脏（连接蛋白 26 和 32）和心脏（连接蛋白 43）间隙连接蛋白的抗体，我们在成年啮齿动物大脑冰冻切片中对特定细胞类型产生了局部免疫反应。在少突胶质细胞和一些神经元中发现了连接蛋白 32 的反应性，而连接蛋白 26 和 43 的反应性则局限于软脑膜细胞、室管膜细胞和松果体。星形胶质细胞中也发生了与连接蛋白 43 抗体的免疫反应性。此外，在脑组织的胚胎和出生后成熟过程中，间隙连接蛋白存在差异表达。连接蛋白 43 和 26 在胚胎大脑的神经上皮中占主导地位，而连接蛋白 32 实际上不存在。出生后 3 至 6 周期间，连接蛋白 26 大部分从未成熟的大脑中消失；这个时间过程对应于连接蛋白 32 表达的增加。连接蛋白 43 的表达在整个胚胎和出生后发育过程中保持高水平。这些发现表明，大脑中间隙连接的表达是多种多样的，特定的细胞类型表达不同的连接蛋白。这种细胞特异性分布可能意味着这些细胞间通道在不同位点和发育阶段的功能存在差异。
By using antibodies directed against gap junction proteins of liver (connexins 26 and 32) and heart (connexin 43), we have localized immunoreactivity to specific cell types in frozen sections of adult rodent brains. Connexin 32 reactivity was found in oligodendrocytes and also in a few neurons, whereas reactivity to connexins 26 and 43 was localized to leptomeningeal cells, ependymal cells, and pineal gland. Immunoreactivity with antibodies to connexin 43 also occurred in astrocytes. Furthermore, during embryonic and postnatal maturation of brain tissues, gap junction proteins were differentially expressed. Connexins 43 and 26 predominated in the neuroepithelium of embryonic brains, whereas connexin 32 was virtually absent. Between 3 and 6 weeks after birth, connexin 26 largely disappeared from immature brain; this time course corresponded to the increased expression of connexin 32. Expression of connexin 43 remained high throughout embryonic and postnatal development. These findings demonstrate that gap junction expression in the brain is diverse, with specific cell types expressing different connexins; this cell-specific distribution may imply differences in the function of these intercellular channels in different loci and developmental stages.