Morphological, immunological, and biochemical analyses of chicken spleen cells transformed in vitro by reticuloendotheliosis virus strain T.
Morphological, immunological, and biochemical analyses of chicken spleen cells transformed in vitro by reticuloendotheliosis virus strain T.
复制标题
网状内皮增生病毒 T 株体外转化鸡脾细胞的形态学、免疫学和生化分析。
作者：
T. Shibuya;I. Chen;A. Howatson;T. W. Mak
网状内皮增生病毒株T （REV-T）是一种高致癌性的禽类逆转录病毒，可引起淋巴网状系统的快速肿瘤疾病。我们从感染REV-T的鸡脾脏细胞中获得了6个细胞系（1- 3,1 - 5,2 -10,2-14,2-16和2-20）。这些细胞可以产生REV-T和相关的网状内皮增生辅助病毒REV-A。这些细胞的组织化学分析表明，虽然它们没有被联苯胺、过氧化物酶、β -葡萄糖醛酸酶或酸性α -乙酸萘酯酶染色，但它们含有很高比例（95%）的酸性磷酸酶阳性细胞。这些细胞的光镜和电镜研究也显示成淋巴细胞或活化淋巴细胞的形态，细胞核不规则，染色质分散。免疫化学分析表明，几乎所有（90% ~ 100%）的细胞都含有表面标记物Ia，但免疫荧光染色未检测到胞质免疫球蛋白M和免疫球蛋白G。结果还表明，其中一些细胞系含有低水平的末端转移酶（0.02至0.17单位/10(9)个细胞），其中一部分（3至35%）细胞可以被针对鸡法氏囊细胞的抗血清染色。这些结果与高致癌性REV-T转化的细胞本质上是淋巴细胞的结论是一致的。此外，这些细胞克隆中至少有一些可能含有活化b淋巴细胞的特征。对这些细胞克隆的分析表明，一些细胞系包含贴壁和非贴壁群体，在形态上存在一些差异。此外，电镜检查显示，虽然非贴壁细胞活跃地产生C型病毒，但在贴壁细胞群中没有或很少出现C型病毒。这些结果支持这样的结论，即其中一些细胞系是异质的，并且包含在产生病毒的能力方面存在差异的细胞亚群。
Reticuloendotheliosis virus strain T (REV-T) is a highly oncogenic avian retrovirus which causes a rapid neoplastic disease of the lymphoreticular system. We derived six cell lines (1-3, 1-5, 2-10, 2-14, 2-16, and 2-20) from chicken spleen cells infected with REV-T. These cells can produce both the REV-T and its associated reticuloendotheliosis helper virus, REV-A. Histochemical analyses of these cells indicate that, while they are not stained by benzidine, peroxidase, beta-glucuronidase or acid alpha-naphthyl acetate esterase, they contain a high proportion (95%) of cells positive for acid phosphatase. Light and electron microscopic studies of these cells also revealed morphologies of lymphoblasts or activated lymphocytes with irregular nuclei and dispersed chromatin. Immunochemical analyses indicate that essentially all (90 to 100%) of the cells contain the surface marker Ia, but no cytoplasmic immunoglobulin M and immunoglobulin G could be detected by immunofluorescence staining. Results also show that some of these cell lines contain a low level of terminal transferase (0.02 to 0.17 unit/10(9) cells), and a proportion (3 to 35%) of these cells can be stained by an antiserum directed against chicken bursa cells. These results are consistent with the conclusion that the cells transformed by the highly oncogenic REV-T are lymphoid in nature. In addition, at least some of these cell clones may contain features characteristic of activated B-lymphocytes. Analysis of these cell clones indicates that some cell lines contain an adherent and nonadherent population with some differences in morphologies. In addition, electron microscopic examination revealed that, while the non-adherent cells are actively producing type C viruses, type C viruses are either absent or very rare in the adherent cell populations. These results support the conclusion that some of these cell lines are heterogeneous and contain subpopulations of cells with differences in their ability to produce viruses.