Chevrier's Field Mouse (Apodemus chevrieri) and PSre David's Vole (Eothenomys melanogaster) in China Carry Orthohepeviruses that form Two Putative Novel Genotypes Within the Species Orthohepevirus C
Chevrier's Field Mouse (Apodemus chevrieri) and PSre David's Vole (Eothenomys melanogaster) in China Carry Orthohepeviruses that form Two Putative Novel Genotypes Within the Species Orthohepevirus C
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中国的田鼠 (Apodemus chevrieri) 和田鼠 (Eothenomys melanogaster) 携带的正肝炎病毒形成了正肝炎病毒 C 种内的两种假定的新基因型。
DOI：
10.1007/s12250-018-0011-8
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发表时间：
2018-02-01
影响因子：
5.5
通讯作者：
Yang, Xing-Lou
中科院分区：
文献类型：
--
作者：
Wang, Bo;Li, Wen;Yang, Xing-Lou
戊型肝炎病毒(HEV)是Hepeviridae家族的原型，也是常见的急性病毒性肝炎的病原体。在多种哺乳动物中发现了基因多样化的HEV相关病毒，其中一些可能具有人畜共患病的潜力。本研究采用广谱逆转录聚合酶链式反应(RT-PCR法)对采自云南省7种野生小型哺乳动物中国的278份标本进行了正型肝炎病毒的检测。在两种啮齿动物中检出HEV相关序列，分别为切氏姬鼠(Apodemus Chevrieri)和黑腹田鼠(Eothenomys Blackogaster)，感染率分别为29.20%(59/202)和7.27%(4/55)。另外还产生了四个具有代表性的全长基因组：雪佛兰田鼠(命名为RdHEVAc14和RdHEVAc86)和PSre David田鼠(命名为RdHEVEm40和RdHEVEm67)各两个基因组。系统发育分析和基因编码区氨基酸序列的两对距离比较表明，在中国雪鼠和大田鼠体内发现的正型病毒属于正型病毒C，但与两个已指定的基因类型有很大的差异：来自大鼠和水貂的HEV-C1型和来自雪貂和可能的水貂的HEV-C2。实时荧光定量RT-PCR结果表明，这些新发现的正型肝炎病毒具有肝嗜性。综上所述，我们的工作在正型病毒C中初步发现了两种可能的新基因型别HEV-C3和HEV-C4，这扩大了我们对啮齿目正型病毒感染的认识，并为正型病毒的起源、进化和宿主范围提供了新的见解。
Hepatitis E virus (HEV) is the prototype of the family Hepeviridae and the causative agent of common acute viral hepatitis. Genetically diverse HEV-related viruses have been detected in a variety of mammals and some of them may have zoonotic potential. In this study, we tested 278 specimens collected from seven wild small mammal species in Yunnan province, China, for the presence and prevalence of orthohepevirus by broad-spectrum reverse transcription (RT)-PCR. HEV-related sequences were detected in two rodent species, including Chevrier's field mouse (Apodemus chevrieri, family Muridae) and PSre David's vole (Eothenomys melanogaster, family Cricetidae), with the infection rates of 29.20% (59/202) and 7.27% (4/55), respectively. Further four representative full-length genomes were generated: two each from Chevrier's field mouse (named RdHEVAc14 and RdHEVAc86) and PSre David's vole (RdHEVEm40 and RdHEVEm67). Phylogenetic analyses and pairwise distance comparisons of whole genome sequences and amino acid sequences of the gene coding regions showed that orthohepeviruses identified in Chinese Chevrier's field mouse and PSre David's vole belonged to the species Orthohepevirus C but were highly divergent from the two assigned genotypes: HEV-C1 derived from rat and shrew, and HEV-C2 derived from ferret and possibly mink. Quantitative real-time RT-PCR demonstrated that these newly discovered orthohepeviruses had hepatic tropism. In summary, our work discovered two putative novel genotypes orthohepeviruses preliminarily named HEV-C3 and HEV-C4 within the species Orthohepevirus C, which expands our understanding of orthohepevirus infection in the order Rodentia and gives new insights into the origin, evolution, and host range of orthohepevirus.