DETERMINATION OF DEOXYRIBONUCLEIC-ACID REPLICATION TIME IN EXPONENTIALLY GROWING ESCHERICHIA-COLI B-R
DETERMINATION OF DEOXYRIBONUCLEIC-ACID REPLICATION TIME IN EXPONENTIALLY GROWING ESCHERICHIA-COLI B-R
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DOI：
10.1128/jb.130.3.1206-1213.1977
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发表时间：
1977-01-01
影响因子：
3.2
通讯作者：
BREMER, H
中科院分区：
文献类型：
--
作者：
CHURCHWARD, G;BREMER, H
在E. coli B/r（ATCC 12407）和该菌株的低胸腺嘧啶需求衍生物。在Thy-菌株中，C作为生长速率和来自逐步增加或氯霉素实验的外源性胸腺嘧啶浓度的函数来测量。在Thy+亲本菌株中，C仅作为生长速率的函数并且仅通过氯霉素方法测量。C周期随着生长速率的增加而减少，并且在Thy-菌株中，C周期也随着胸腺嘧啶浓度的增加而减少。在高生长速率下观察到约37 min的值。
The time necessary to replicate the chromosome (C period) was measured in E. coli B/r (ATCC 12407) and a low-thymine-requiring derivative of that strain. In the Thy- strain, C was measured as a function of growth rate and exogenous thymine concentration from step-up or chloramphenicol experiments. In the Thy+ parental strain, C was measured only as a function of the growth rate and only by the chloramphenicol method. The C period decreased with growth rate and, in the Thy- strain, the C period also decreased with increasing thymine concentration. A value of about 37 min was observed at high growth rates.