Post‐Irradiation Thymocyte Regeneration After Bone Marrow Transplantation I. Regeneration and Quantification of Thymocyte Progenitor Cells In the Bone Marrow
Post‐Irradiation Thymocyte Regeneration After Bone Marrow Transplantation I. Regeneration and Quantification of Thymocyte Progenitor Cells In the Bone Marrow
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骨髓移植后辐照后胸腺细胞再生 I. 骨髓中胸腺细胞祖细胞的再生和定量
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
G. Westen
中科院分区：
文献类型：
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作者：
W. Boersma;I. Betel;R. Daculsi;G. Westen
将骨髓移植到经辐照的同系受体小鼠后，供体型胸腺细胞群的生长动力学呈双相性。在第一个快速再生阶段，持续到移植后第19天，供体细胞的发育速率与接种的骨髓细胞数量无关。第二慢相仅在移植少量骨髓细胞（2.5 × 104）时观察到。发育速率的降低归因于供体细胞从胸腺流出，因为同时在脾脏中发现了第一个免疫活性细胞。骨髓移植后，与CFU的再生相比，骨髓中胸腺祖细胞的再生延迟。因此，再生骨髓具有大大降低的能力，以恢复胸腺细胞群。移植3 × 106个细胞后1周，骨髓再生量仅为正常骨髓容量的1%，说明骨髓移植后再生的胸腺细胞群是由接种物中的前体细胞的直接后代组成。
Growth kinetics of the donor‐type thymus cell population after transplantation of bone marrow into irradiated syngeneic recipient mice is biphasic. During the first rapid phase of regeneration, lasting until day 19 after transplantation, the rate of development of the donor cells is independent of the number of bone marrow cells inoculated. the second slow phase is observed only when low numbers of bone marrow cells (2.5 × 104) are transplanted. the decrease in the rate of development is attributed to an efflux of donor cells from the thymus because, at the same time, the first immunologically competent cells are found in spleen. After bone marrow transplantation the regeneration of thymocyte progenitor cells in the marrow is delayed when compared to regeneration of CFUs. Therefore, regenerating marrow has a greatly reduced capacity to restore the thymus cell population. One week after transplantation of 3 × 106 cells, 1% of normal capacity of bone marrow is found. It is concluded that the regenerating thymus cells population after bone marrow transplantation is composed of the direct progeny of precursor cells in the inoculum.