WESTERN BLOTTING - ELECTROPHORETIC TRANSFER OF PROTEINS FROM SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELS TO UNMODIFIED NITROCELLULOSE AND RADIOGRAPHIC DETECTION WITH ANTIBODY AND RADIOIODINATED PROTEIN-A
WESTERN BLOTTING - ELECTROPHORETIC TRANSFER OF PROTEINS FROM SODIUM DODECYL SULFATE-POLYACRYLAMIDE GELS TO UNMODIFIED NITROCELLULOSE AND RADIOGRAPHIC DETECTION WITH ANTIBODY AND RADIOIODINATED PROTEIN-A
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DOI：
10.1016/0003-2697(81)90281-5
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发表时间：
1981-01-01
影响因子：
2.9
通讯作者：
BURNETTE, WN
中科院分区：
文献类型：
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作者：
BURNETTE, WN
采用一种简单而有效的方法将蛋白质从十二烷基硫酸钠-聚丙烯酰胺凝胶转移到纯净的、未经修饰的硝酸纤维素片上。然后，通过与特定抗体的反应以及随后放射性碘标记的葡萄球菌蛋白A与免疫复合体的结合，固定化的蛋白质可以在放射学上原位可视化。复杂细胞裂解物中小鼠白血病病毒抗原的检测[C57BL/6 E.用G2细胞和C3H细胞]验证了该技术的有效性。
A simple and efficient procedure was employed for the electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to sheets of pure, unmodified nitrocellulose. Immobilized proteins could then be radiographically visualized in situ by reaction with specific antibody and the subsequent binding of radioiodinated Staphylococcus protein A to the immune complexes. The detection of murine leukemia virus antigens in complex cellular lysates [C57BL/6 E.male. G2 cells and C3H cells] was used to demonstrate the efficacy of this technique.