The next generation cell-penetrating peptide and carbon dot conjugated nano-liposome for transdermal delivery of curcumin (Retracted Article)
The next generation cell-penetrating peptide and carbon dot conjugated nano-liposome for transdermal delivery of curcumin (Retracted Article)
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DOI：
10.1039/c5bm00433k
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发表时间：
2016-01-01
影响因子：
6.6
通讯作者：
Sharma, Prashant K.
中科院分区：
文献类型：
--
作者：
Patra, Santanu;Roy, Ekta;Sharma, Prashant K.
为克服传统脂质体透皮给药存在的透皮能力有限、稳定性差等问题，本文报道了新一代含细胞穿透肽聚精氨酸的碳量子点纳米脂质体。新合成的，具有成本效益的脂质体前体用于脂质体的制造。所得脂质体具有与常规脂质体类似的双层结构，具有小得多的尺寸、更高的稳定性和高渗透能力。纳米脂质体在室温下储存三个月后显示出高稳定性，尺寸或包封率没有任何变化。碳量子点的掺入也开辟了它们在荧光细胞成像研究中的应用，这得到了脂质体皮肤渗透的荧光显微镜分析的很好支持。所制备的纳米脂质体即使在高浓度下也不显示对MCF-7细胞的任何细胞毒性;然而，当应用载药脂质体时，它们可以以高速率杀死癌细胞。所合成的纳米脂质体有望成为一种高效、稳定、生物相容性好的经皮给药纳米载体。
To overcome the problems associated with conventional liposomes in transdermal drug delivery like limited penetration ability and poor stability, in this article we report a new generation of cell penetrating peptide polyarginine containing nano-liposomes conjugated with carbon dots. The newly synthesized, cost-effective liposomic precursors were used for the fabrication of liposomes. The resulting liposomes have a bilayer structure like that of conventional liposomes with much smaller size, higher stability, and high penetration ability. The nano-liposomes show high stability at room temperature for three months without any change in size or encapsulation efficiency. The incorporation of carbon dots also opens up their application in fluorescence cell imaging studies, which is very well supported by the fluorescence microscopic analysis of the liposome skin penetration. The as-prepared nano-liposomes do not show any cytotoxicity for MCF-7 cells, even at high concentrations; however, when drug loaded liposomes are applied, they can kill the cancer cells with a high rate. The synthesized nano-liposomes have the potential to be used as an efficient, stable, biocompatible nanocarrier for transdermal drug delivery.