SUBSTANCE P-IMMUNOREACTIVE RETINAL GANGLION-CELLS AND THEIR CENTRAL AXON TERMINALS IN THE RABBIT
SUBSTANCE P-IMMUNOREACTIVE RETINAL GANGLION-CELLS AND THEIR CENTRAL AXON TERMINALS IN THE RABBIT
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DOI：
10.1038/327155a0
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发表时间：
1987-05-14
期刊：
影响因子：
64.8
通讯作者：
LIEBERMAN, AR
中科院分区：
文献类型：
--
作者：
BRECHA, N;JOHNSON, D;LIEBERMAN, AR
视网膜神经节细胞是连接视网膜和大脑的投射神经元。哺乳动物视网膜节细胞层(GCL)中的多肽免疫反应细胞已被发现，但它们的身份尚未确定1-3。我们现在报告，在兔的所有视网膜神经节细胞中，25-35%的细胞含有P物质(SP)免疫反应。通过上丘注射的荧光示踪剂的逆行运输或视神经切断术后的逆行变性来鉴定它们。SP免疫反应阳性细胞存在于视网膜的所有部分，具有中到大的细胞体，树突广泛分布于近端的内丛状层。它们的轴突终止于膝状背外侧核、上丘和副视核，在对侧视神经切断和/或眼球摘除后这些终末完全消失。在外侧膝状核背侧，大的珠状免疫反应阳性轴突和突起在视束正下方组成一个狭窄的神经丛，在那里它们定义了一个新的膝曲板层。这些突触与膝状背外侧核的树突、投射神经元和复杂的肾小球神经束内的中间神经元发生多次突触接触。这是一些哺乳动物视网膜神经节细胞含有P物质样肽的直接证据，并有力地表明，在兔体内，P物质(或相关的速激肽)可能是特定群体或群体的视网膜神经节细胞的递质或调节剂。
Retinal ganglion cells are the projection neurons that link the retina to the brain. Peptide immunoreactive cells in the ganglion cell layer (GCL) of the mammalian retina have been noted but their identity has not been determined1–3. We now report that, in the rabbit, 25–35% of all retinal ganglion cells contain substance P-like (SP) immunoreactivity. They were identified by either retrograde transport of fluorescent tracers injected into the superior colliculus, or by retrograde degeneration after optic nerve section. SP immunoreactive cells are present in all parts of the retina and have medium to large cell bodies with dendrites that ramify extensively in the proximal inner plexiform layer. Their axons terminate in the dorsal lateral geniculate nucleus, superior colliculus and accessory optic nuclei, and these terminals disappear completely after contralateral optic nerve section and/or eye enucleation. In the dorsal lateral geniculate nucleus large, beaded, immunoreactive axons and varicosities make up a narrow plexus just below the optic tract, where they define a new geniculate lamina. The varicosities make multiple synaptic contacts with dendrites of dorsal lateral geniculate nucleus projection neurons and presumptive interneurons in complex glomerular neuropil. This is direct evidence that some mammalian retinal ganglion cells contain substance P-like peptides and strongly suggests that, in the rabbit, substance P (or related tachykinins) may be a transmitter or modulator in a specific population or populations of retinal ganglion cells.