Developmental toxicity and transcriptome analysis of zebrafish (Danio rerio) embryos following exposure to chiral herbicide safener benoxacor
Developmental toxicity and transcriptome analysis of zebrafish (Danio rerio) embryos following exposure to chiral herbicide safener benoxacor
复制标题
斑马鱼（Danio rerio）胚胎接触手性除草剂安全剂 Benoxacor 后的发育毒性和转录组分析
DOI：
10.1016/j.scitotenv.2020.143273
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发表时间：
2021-01-14
影响因子：
9.8
通讯作者：
Bai, Lianyang
中科院分区：
文献类型：
--
作者：
Liu, Sihong;Deng, Xile;Bai, Lianyang
已在溪流中检测到S-异丙甲草胺的手性除草剂安全剂苯恶唑。然而，这对水生生态系统构成的潜在风险尚不清楚。本研究以斑马鱼(Danio Rerio)胚胎为模型，研究了苯恶唑的对映选择性毒性及其对受精后2~96h生物活性和发育的影响。结果表明，苯恶唑对孵化率、畸形率和死亡率有负面影响。与单个对映体相比，RAc-Benoxacor暴露的胚胎具有更高的急性毒性和发育毒性、谷胱甘肽S转移酶(GST)和谷胱甘肽过氧化物酶(GPx)活性以及NRF2的表达水平。它们的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)活性较低，KRT17、Tbx16、OSx、CAT、bcl2、bax和干扰素的表达水平较低。高通量RNA测序显示，RAC-Benoxacor对基因调控的影响比任何一种对映体都要大。基因本体论丰富和京都百科全书的基因和基因组途径分析表明，氧化还原酶活性、细胞脂质代谢过程和催化活性相关基因的变化可能是由于苯恶唑异构体的对映选择性作用。这些结果表明，在未来的环境风险评价中，应考虑手性苯恶唑对斑马鱼影响的生态毒理学数据和安全知识。(C)2020爱思唯尔B.V.保留所有权利。
Benoxacor, a chiral herbicide safener for S-metolachlor, has been detected in streams. However, the potential risk this poses to aquatic ecosystems is not clear. This study used zebrafish (Danio rerio) embryos as a model to assess the enantioselective toxicity of benoxacor and its effects on biological activity and development from 2 h to 96 h post-fertilization (hpf). Results showed that benoxacor had negative effects on hatchability, malformations, and mortality. Compared to either individual enantiomer, embryos exposed to Rac-benoxacor had higher acute and developmental toxicities, glutathione S-transferase (GST) and glutathione peroxidase (GPx) enzyme activities, and nrf 2 expression levels. They also had lower superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GR) enzyme activity and krt 17, tbx 16, osx, cat, bcl 2, bax, and ifn expression levels. High-throughput RNA sequencing revealed that Rac-benoxacor had a greater effect on gene regulation than either enantiomer. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analyses demonstrated that changes in oxidoreductase activity, cellular lipid metabolic process, and catalytic activity related genes may be due to the enantioselective effects of benoxacor isomers. These results suggest that the ecotoxicology data and safety knowledge about the effects of chiral benoxacor on zebrafish should be considered in future environmental risk evaluation. (C) 2020 Elsevier B.V. All rights reserved.