Enhancement of presynaptic calcium current by cysteine string protein
Enhancement of presynaptic calcium current by cysteine string protein
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DOI：
10.1113/jphysiol.2001.013397
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发表时间：
2002-01-15
影响因子：
5.5
通讯作者：
Stanley, EF
中科院分区：
文献类型：
--
作者：
Chen, S;Zheng, X;Stanley, EF
用离体鸡睫状神经元的萼突触制备物来检测半胱氨酸串蛋白（CSP）对突触前N型Ca ~（2+）通道的作用。内源性CSP主要定位于突触前神经末梢的分泌囊泡簇。将重组CSP引入电压钳位末端导致Ca 2+电流幅度显著增加。然而，这种增加不能归因于Ca 2+通道动力学的变化，电压依赖性，prepulseinactivation，或G蛋白抑制，但归因于休眠通道的招聘。分泌囊泡相关的内源性CSP可能在增强递质释放部位的Ca 2+通道活性中起重要作用。
The isolated chick ciliary neuron calyx synapse preparation was used to test cysteine string protein (CSP) action on presynaptic N-type Ca2+ channels. Endogenous CSP was localized primarily to secretory vesicle clusters in the presynaptic nerve terminal. Introduction of recombinant CSP into the voltage clamped terminal resulted in a prominent increase in Ca2+ current amplitude. However, this increase could not be attributed to a change in Ca2+ channel kinetics, voltage dependence, prepulseinactivation, or G protein inhibition but was attributed to the recruitment of dormant channels. Secretory vesicle associated endogenous CSP may play an important role in enhancing Ca2+ channel activity at the transmitter release site.