Immobilization of Glucose Oxidase by Electropolymerization of Monomers
Immobilization of Glucose Oxidase by Electropolymerization of Monomers
复制标题
单体电聚合固定化葡萄糖氧化酶
DOI：
10.1111/j.1749-6632.1990.tb18198.x
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发表时间：
1990
影响因子：
5.2
通讯作者：
Mark K. Malmros
中科院分区：
文献类型：
--
作者：
N. F. Almeida;L. B. Wingard;Mark K. Malmros
酶在电子设备中大规模应用的缺点之一是难以制造均匀、可重复的电路。与在非常小的区域上提供空间均匀的酶涂层相关的问题在很大程度上被忽略了。为了开发分析设备并强调可能的体内使用的小型化，设计中的一个关键因素是能够在换能器元件上具有功能均匀且可重复的酶负载。聚合物改性电极具有在非常小的区域上提供均匀涂层的明显优势，特别是通过电聚合原位形成时。蛋白质在聚合物涂层内的固定可以通过聚合物基质内的包埋或可能通过共价连接至官能化单体来实现。这项工作旨在研究电聚合条件对固定化酶的负载、空间分布和活性的影响，使用导电聚合物（如吡咯）和非导电聚合物[如聚对苯二胺]和不同类型的酶。给出了间苯二胺和葡萄糖氧化酶的结果。
One of the drawbacks to large-scale applications of enzymes in electronic devices has been the difficulty in fabricating uniform, reproducible circuitry. The problems associated with providing spatially uniform coatings of enzymes over very small areas have for the most part been ignored. With the aim of developing analytical devices and with an emphasis on miniaturization for possible in vivo use, a critical factor in the design is the ability to have functionally uniform and reproducible enzyme loadings on the transducer element. Polymer-modified electrodes offer the apparent advantage of providing uniform coatings over very small areas, particularly when formed in situ by electropolymerization. Immobilization of protein within the polymer coating can be by entrapment within the polymer matrices'-' or possibly by covalent attachment to functionalized monomers. This work is directed at studying the effect of the conditions of electropolymerization on the loading, spatial distribution, and activity of the immobilized enzyme, using both conducting polymers (such as pyrrole) and nonconducting polymers [such as poly(rn-phenylenediamine)] and different types of enzymes. Results are presented for m-phenylenediamine and glucose oxidase.