Tumor necrosis factor-α expression in muscles of polymyositis and dermatomyositis
Tumor necrosis factor-α expression in muscles of polymyositis and dermatomyositis
复制标题
DOI：
10.1007/s004010051165
复制
发表时间：
2000-05-01
影响因子：
12.7
通讯作者：
Sobue, G
中科院分区：
文献类型：
--
作者：
Kuru, S;Inukai, A;Sobue, G
检测多发性肌炎(PM)和皮肌炎(DM)患者肌肉活检标本中肿瘤坏死因子-α(TNF-α)的表达，探讨其在PM和DM发病机制中的作用。我们对10例PIM和5例DM患者的肌肉进行了肿瘤坏死因子-α的非放射性原位杂交研究和细胞类型特异性标记的免疫组织化学研究。在所有PM和DM肌肉中，肌内膜和肌周膜均可见肿瘤坏死因子-α阳性细胞。PM组和DM组肿瘤坏死因子α阳性细胞占总浸润细胞的比例相似(PM组27.1+/-7.4%，DM组28.5+/-13.6%)。而非坏死性肌纤维内的肿瘤坏死因子α/CD8阳性淋巴细胞和肿瘤坏死因子α阳性巨噬细胞仅见于PM组，DM组未见。在PM和DM中，肿瘤坏死因子-α的表达比先前认为的更高，提示肿瘤坏死因子-α在PM的肌纤维变性中起一定作用。
We evaluated the expression of tumor necrosis factor-alpha (TNF-alpha) mRNA in muscle biopsy specimens from patients with polymyositis (PM) and dermatomyositis (DM) to clarify its role in the pathogenesis of PM and DM. We performed non-radioactive in situ hybridization studies for TNF-alpha combined with immunohistochemistry for cell type-specific markers on muscles from ten PIM and five DM patients. TNF-alpha-positive infiltrating cells present in the endomysium and perimysium were found in all PM and DM muscles. The frequency of TNF-alpha-positive cells against total infiltrating cells was similar among PM and DM (27.1 +/- 7.4% in PM and 28.5 +/- 13.6% in DM). However, TNF-alpha/CD8-positive lymphocytes and TNF-alpha-positive macrophages invading the non-necrotic muscle fiber were observed only in PM but not in DM. TNF-alpha was more highly expressed in PM and DM than was previously thought, and it was suggested that TNF-alpha plays a role in muscle fiber degeneration in PM.