Genetic relationships among Pistacia species using AFLP markers
Genetic relationships among Pistacia species using AFLP markers
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DOI：
10.1007/s00606-008-0117-9
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发表时间：
2009-04
影响因子：
1.9
通讯作者：
H. Karimi;S. Kafkas;Z. Zamani;A. Ebadi;Mohamad Reza Fatahi Moghadam
中科院分区：
文献类型：
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作者：
H. Karimi;S. Kafkas;Z. Zamani;A. Ebadi;Mohamad Reza Fatahi Moghadam
本研究利用扩增片段长度多态(AFLP)技术研究了两个新种之间的亲缘关系。本研究的植物材料包括44份属于TOP的材料。Vera，P.eurycarpa，P.khinjuk，均为OFP亚种。Atlantica(Atlantica，mutica，kurdica和Cabulica)，三个未知的基因类型和三个材料，被认为是由P.这些材料来自伊朗、土耳其、美国和叙利亚。6对AFLP引物组合共扩增出475条带，平均每对扩增出79.16条带，其中336条为多态条带。对Jaccard相似系数矩阵和各物种的平均相似性进行基于算术平均的未加权配对组法(UPGMA)分析。根据结果，形成了两个主要的聚类群和P。Vera、P.eurycarpa、P.Atlantica(atlantica亚种、kurdica亚种、mutica、Cabulica亚种)和杂交种类型位于第一主类群，来自伊朗和美国的P.khinjukaccus品种位于第二主类群。确认了野生种与野生种之间的杂交种以及它们的杂交性质。其中一个未知种质与杂交种聚在一起，另外两个与P.金珠。根据这项研究，距离最近的物种最多。Verais Eurycarpa组，BYP组。亚特兰蒂卡。UPGMA分析分离出P。Atlanticassubbsp.mutica.comulicae来自P.大西洋和太平洋。真果树。亚种MUTICA和CABLICA是两个最接近的基因型别，因此PATLATLANICA亚种MUTICICA可以被归类为一个不同的种。MULTICA和TECURICIA为OFP的一个亚种。慕蒂卡。本研究表明，P。Eurycarpai是P的代名词。Atlanticassubbsp.kurdica，应被认为与P.Atlantica；然而，P.atlanticas的遗传相似性最高。Eurycarpa比其他物种都要好。
This study addresses the phylogenetic relationship betweenPistaciaspecies by amplified fragment length polymorphism (AFLP). The plant materials of this study consisted of a total of 44 accessions belonging toP. vera,P. eurycarpa,P. khinjuk, all subspecies ofP. atlantica(atlantica,mutica,kurdicaandcabulica), three unknown genotypes and three accessions, proposed to be hybrid fromP. eurycarpa×P. atlantica.The accessions were from Iran, Turkey, USA and Syria. Six AFLP primer combinations produced a total of 475 fragments, with average of 79.16 fragments per primer pair, of which, 336 bands were polymorphic. Unweighted pair group method based on arithmetic average (UPGMA) analysis was performed on jaccard’s similarity coefficient matrix and also average similarity of each species. According to the results, two main clusters were developed andP. vera,P. eurycarpa,P. atlantica(subsp.atlantica,kurdica,mutica,cabulica) and the hybrid genotypes located in the first main cluster.P. khinjukaccessions from Iran and USA localized in second main cluster. The hybrid accessions located betweeneurycarpaandatlanticaspecies and their hybrid nature between these two species were confirmed. One of the unknown accessions clustered with the hybrid ones and the two other were grouped closely withP. Khinjuk. According to this study, the closest species toP. verawas Eurycarpa group, followed byP. atlantica. UPGMA analysis separatedP. atlanticasubsp.muticaandcabulicafromP. atlanticaandP. eurycarpa. Subspeciesmuticaandcabulicawere two closest genotypes; hence,P. atlanticasubsp.muticacould be classified as a distinct species asP. muticaand thecabulicaas a subspecies ofP. mutica. This study revealed thatP. eurycarpais synonym forP. atlanticasubsp.kurdicaand should be considered distinct fromP. atlantica; however,P. atlanticashowed a closer genetic similarity toP. eurycarpathan the other species.