Biocompatibility of Two Novel Root Repair Materials
Biocompatibility of Two Novel Root Repair Materials
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DOI：
10.1016/j.joen.2011.02.029
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发表时间：
2011-06-01
影响因子：
4.2
通讯作者：
Haapasalo, Markus
中科院分区：
文献类型：
--
作者：
Ma, Jingzhi;Shen, Ya;Haapasalo, Markus
简介：本研究的目的是评价2种根端充填材料Endosequence牙根修复材料腻子（ERRM Putty）和糊剂（ERRM Paste）的生物相容性，并与灰色矿物三氧化物聚合物（MTA）进行比较。方法：对ERRM腻子、ERRM糊剂、MTA、中间体修复材料和Cavit G进行测试。对于细胞毒性试验，将人牙龈成纤维细胞与来自设定为2天或7天的材料的不同浓度的浸提液孵育1、3和7天。通过甲基噻唑四氮唑（MTT）测定法评价细胞活力。对于细胞粘附试验，在凝固后立即、在细胞培养基中孵育7天后以及在牙龈成纤维细胞悬液中以5 × 10（4）个细胞/孔的密度孵育2天和7天后，在扫描电子显微镜下检查凝固7天的材料。用X射线能谱分析确定了材料表面形成的晶体成分。结果：细胞存活率与材料种类、凝固时间、孵育时间相关（P
Introduction: The purpose of the present study was to evaluate the biocompatibility of 2 root-end filling materials, Endosequence Root Repair Material Putty (ERRM Putty) and Paste (ERRM Paste) and compare them with gray mineral trioxide aggregate (MTA). Methods: ERRM Putty, ERRM Paste, MTA, intermediate restorative material (IRM), and Cavit G were tested. For cytotoxicity assay, human gingival fibroblasts were incubated for 1, 3, and 7 days with extracts of varying concentrations from materials set for 2 days or 7 days. Cell viability was evaluated by methyl-thiazol-tetrazolium (MTT) assay. For cell adhesion assay, materials set for 7 days were examined under scanning electron microscope directly after setting, after incubation in cell culture medium for 7 days, and after incubation in gingival fibroblast suspension at a density of 5 x 10(4) cells/well for 2 and 7 days. The constituents of crystals formed on surface of materials were determined by energy dispersive analysis by x-ray. Results: Cell viability was significantly correlated with the type of material, setting time, and incubation time (P