Purification of chicken intestinal receptor for 1 alpha, 25-dihydroxyvitamin D3 to apparent homogeneity.
Purification of chicken intestinal receptor for 1 alpha, 25-dihydroxyvitamin D3 to apparent homogeneity.
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纯化鸡肠道受体 1 α, 25-二羟基维生素 D3 至明显均质。
DOI：
10.1073/pnas.79.1.16
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发表时间：
1982
影响因子：
11.1
通讯作者：
DeLuca,HF
中科院分区：
文献类型：
--
作者：
Simpson,RU;DeLuca,HF
鸡肠道1 α， 25-二羟基维生素D3受体样蛋白经十二烷基硫酸钠凝胶电泳纯化，具有明显的同质性。用于纯化的技术包括Polymin P（聚乙烯亚胺）和（NH4）2SO4选择性沉淀受体，羟基磷灰石的批量吸附和选择性洗脱，然后是凝胶排斥和deae -纤维素层析。最后，标记的受体在pH约为6.0的色谱聚焦柱上洗脱。该蛋白被纯化6100倍，以9%的收率获得受体。
The chicken intestinal 1 alpha, 25-dihydroxyvitamin D3 receptor-like protein has been purified to apparent homogeneity as determined by sodium dodecyl sulfate gel electrophoresis. The techniques employed for the purification include selective precipitation of the receptor by Polymin P (polyethyleneimine) and (NH4)2SO4 and batch adsorption to and selective elution from hydroxylapatite, followed by gel exclusion and DEAE-cellulose chromatography. Finally, the labeled receptor was eluted at a pH of approximately 6.0 on a chromatofocusing column. The protein was purified 6100-fold and the receptor was obtained in 9% yield.