Micropatterning of living cells on a heterogeneously wetted surface
Micropatterning of living cells on a heterogeneously wetted surface
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DOI：
10.1021/la061602k
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发表时间：
2006-09-12
期刊：
影响因子：
3.9
通讯作者：
Allbritton, Nancy L.
中科院分区：
文献类型：
--
作者：
Wang, Yuli;Sims, Christopher E.;Allbritton, Nancy L.
我们报告了一种简单的方法来制造非均匀润湿的表面，可用于图案化活细胞或生物分子。在玻璃表面上制备了由SU-8组成的分子阵列，然后用疏水性硅烷对其进行衍生化。在将水溶液添加到阵列中时，空气被捕获在两个电极之间的疏水空腔内。被困的空气形成了一堵“虚拟墙”，阻挡了进入这些空腔的通道。在阵列上培养的细胞被迫仅在细胞的顶部生长，即，所述表面被水性介质润湿。虚拟墙壁在操纵阵列期间和长时间（数月）内是稳定的。
We report a simple approach to fabricate heterogeneously wetted surfaces which can be used to pattern living cells or biomolecules. An array of pedestals composed of SU-8 was fabricated on a glass surface which was then derivatized with a hydrophobic silane. Upon addition of aqueous solutions to the array, air was trapped within the hydrophobic cavities between the pedestals. The trapped air formed a "virtual wall" blocking access to these cavities. Cells cultured on the array were forced to grow only on the tops of the pedestals, i.e., the surfaces wetted by aqueous media. The virtual walls were stable during manipulation of the array and over long time periods (months).