Genomic diversity of enterohemorrhagic Escherichia coli O157 revealed by whole genome PCR scanning
Genomic diversity of enterohemorrhagic Escherichia coli O157 revealed by whole genome PCR scanning
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DOI：
10.1073/pnas.262441699
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发表时间：
2002-12-24
影响因子：
11.1
通讯作者：
Hayashi, T
中科院分区：
文献类型：
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作者：
Ohnishi, M;Terajima, J;Hayashi, T
肠出血性大肠杆菌O157是全球主要的公共卫生问题之一，引起出血性结肠炎的大爆发以及许多小爆发和散发病例。O157基因组的限制性内切酶消化模式的变异性，这是广泛用于区分菌株的O157感染的分子流行病学，表明菌株之间存在一些基因组多样性。基于O157酒井的全基因组序列，我们分析了8个O157菌株的全基因组结构，显示不同的XbaI消化模式，我们称之为全基因组PCR扫描的系统PCR分析。该分析不仅确定了菌株之间高度保守的O157特异性序列，而且还揭示了出乎意料的高度基因组多样性。特别是，原噬菌体，包括滋贺毒素转导的噬菌体，表现出广泛的结构和位置的多样性，这意味着噬菌体的变化是一个主要因素，在产生基因组多样性之间的O157谱系。
Enterohemorrhagic Escherichia coli O157 is one of the leading worldwide public health concerns, causing large outbreaks of hemorrhagic colitis as well as numerous small outbreaks and sporadic cases. The variability of restriction enzyme-digestion patterns of O157 genomes, which is widely used to distinguish strains in the molecular epidemiology of O157 infections, suggests the presence of some genomic diversity among the strains. Based on the complete genome sequence of O157 Sakai, we analyzed the whole genome structures of eight O157 strains displaying diverse Xbal-digestion patterns by a systematic PCR analysis that we have named whole genome PCR scanning. This analysis identified not only the O157-specific sequences that are highly conserved among the strains, but also revealed an unexpectedly high degree of genomic diversity. In particular, prophages, including Shiga toxin-transducing phages, exhibited extensive structural and positional diversity, implying that variation of bacteriophages is a major factor in generating genomic diversity among the O157 lineage.