Spatiotemporal coordination of stem cell commitment during epidermal homeostasis.
Spatiotemporal coordination of stem cell commitment during epidermal homeostasis.
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DOI：
10.1126/science.aaf7012
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发表时间：
2016-06-17
期刊：
影响因子：
--
通讯作者：
Greco V
中科院分区：
文献类型：
--
作者：
Rompolas P;Mesa KR;Kawaguchi K;Park S;Gonzalez D;Brown S;Boucher J;Klein AM;Greco V
成人组织通过干细胞库取代丢失的细胞。然而，细胞自我更新，承诺和功能整合到组织中的机制仍然没有解决。在活体小鼠中使用成像技术，我们捕获了耳朵和爪子表皮中单个细胞的寿命。我们的数据表明，表皮干细胞具有分裂或直接分化的同等潜力。跟踪多代干细胞表明，细胞行为在代与代之间并不协调。然而，同胞细胞的命运和寿命是耦合的。我们没有观察到受调控的不对称细胞分裂。最后，我们证明了分化干细胞整合到预先存在的表皮有序的空间单位。这项研究阐明了组织是如何通过干细胞行为的时空协调来维持的。
Adult tissues replace lost cells via pools of stem cells. However, the mechanisms of cell self-renewal, commitment, and functional integration into the tissue remain unsolved. Using imaging techniques in live mice, we captured the lifetime of individual cells in the ear and paw epidermis. Our data suggest that epidermal stem cells have equal potential to either divide or directly differentiate. Tracking stem cells over multiple generations reveals that cell behavior is not coordinated between generations. However, sibling cell fate and lifetimes are coupled. We did not observe regulated asymmetric cell divisions. Lastly, we demonstrated that differentiating stem cells integrate into preexisting ordered spatial units of the epidermis. This study elucidates how a tissue is maintained by both temporal and spatial coordination of stem cell behaviors.