Single-cell elemental analysis via high irradiance femtosecond laser ionization time-of-flight mass spectrometry.
Single-cell elemental analysis via high irradiance femtosecond laser ionization time-of-flight mass spectrometry.
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DOI：
10.1021/ac400317q
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发表时间：
2013-04
影响因子：
7.4
通讯作者：
Yao Gao;Yiming Lin;Bochao Zhang;D. Zou;Miaohong He;Bin Dong;W. Hang;Benli Huang
中科院分区：
文献类型：
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作者：
Yao Gao;Yiming Lin;Bochao Zhang;D. Zou;Miaohong He;Bin Dong;W. Hang;Benli Huang
在现代生物分析中，由于复杂的生物基质和元素含量，单细胞的元素分析是重要的，但具有挑战性。我们开发了高辐照度飞秒激光电离正交飞行时间质谱（fs-LI-O-TOFMS）来确定单个细胞的元素组成。通过加热和干燥细胞，样品制备过程简单快速。在典型的操作条件下，可以在具有合理同位素比的光谱中清楚地观察到单个池中的毫微微克级以上的元素。一些用ICPMS难以测定的非金属元素，如P、S和Cl，可以很容易地用fs-LI-O-TOFMS测定。重复分析表明，金属元素的信号变化为15-35%，非金属元素的信号变化为25-50%。结果表明，fs-LI-O-TOFMS是一种简单、快速、实用的单细胞元素测定工具。
In modern bioanalytics, elemental analysis of single cells is important yet challenging due to the complicated biological matrices and elemental contents. We have developed the high irradiance femtosecond laser ionization orthogonal time-of-flight mass spectrometry (fs-LI-O-TOFMS) to determine the elemental composition of individual cells. The sample preparation procedure is simple and fast through heating and drying the cells. Under typical operating conditions, elements above femtogram levels in a single cell can be clearly observed in the spectrum with reasonable isotope ratios. Some of the nonmetallic elements that are difficult to measure by ICPMS, such as P, S, and Cl, can be easily determined by fs-LI-O-TOFMS. Replicate analyses show that signal variations are 15-35% for metallic elements and 25-50% for nonmetallic elements. The results demonstrate that fs-LI-O-TOFMS is a simple, rapid, and practical tool for the elemental determination of single cells.