DECREASE OF SUBSTANCE-P IN PRIMARY AFFERENT NEURONS AND IMPAIRMENT OF NEUROGENIC PLASMA EXTRAVASATION BY CAPSAICIN
DECREASE OF SUBSTANCE-P IN PRIMARY AFFERENT NEURONS AND IMPAIRMENT OF NEUROGENIC PLASMA EXTRAVASATION BY CAPSAICIN
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DOI：
10.1111/j.1476-5381.1980.tb10409.x
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发表时间：
1980-01-01
影响因子：
7.3
通讯作者：
LEMBECK, F
中科院分区：
文献类型：
--
作者：
GAMSE, R;HOLZER, P;LEMBECK, F
1用辣椒素（50 mg/kg，s.c.）在出生后的第二天、第十天或第二十天。三个月后，在皮肤、感觉神经和中枢神经系统中测定免疫反应性P物质（I-SP）。还检查了神经源性血浆外渗。2 2日龄和10日龄预处理可使皮肤、隐神经、迷走神经、背根、脊髓后半段和延髓的I-SP降低26 ~ 69%。脊髓腹侧、中脑、下丘脑、纹状体、皮质和小脑的I-SP含量保持不变。神经源性血浆外渗被抑制超过80%。3与辣椒素对新生大鼠的这种不可逆作用相反，20日龄大鼠的预处理导致I-SP的可逆性耗竭和神经源性血浆外渗的可逆性损害。4.辣椒素预处理可引起大鼠后爪皮肤中I-SP的明显耗竭和神经源性血浆外渗的损害。在皮肤慢性失神经支配后，I-SP也出现类似的下降。5动脉内灌注P物质（阈剂量5 × 10-13 mol/min）或毒蜥毒蛋白（physalaemin）均引起剂量依赖性血浆外渗。生长抑素、血管活性肠多肽、雨蛙肽和脑啡肽类似物FK 33-824在100倍以上的剂量下无效。6结果表明辣椒素对P物质神经元的作用仅限于初级感觉神经元。由于在每一种情况下，P物质的皮肤含量减少与受损的神经源性血浆外渗，这表明P物质的释放参与神经源性血浆外渗。
1 Rats were pretreated with capsaicin (50 mg/kg, s.c.) on the 2nd, 10th, or 20th day of life. Three months later immunoreactive substance P (I—SP) was determined in skin, sensory nerves and the central nervous system. Neurogenic plasma extravasation was also examined. 2 Pretreatment at the age of 2 or 10 days resulted in a decrease (26 to 69%) of I—SP in skin, saphenous and vagus nerve, dorsal roots, dorsal half of the spinal cord, and medulla oblongata. The I—SP content of the ventral half of the spinal cord, of midbrain, hypothalamus, striatum, cortex, and cerebellum remained unchanged. Neurogenic plasma extravasation was inhibited by more than 80%. 3 In contrast to this irreversible effect of capsaicin on newborn rats, pretreatment of 20 day old rats led to reversible depletion of I—SP and to reversible impairment of neurogenic plasma extravasation. 4 Capsaicin pretreatment of adult rats caused a marked depletion of I—SP in the skin of the hind paw and an impairment of neurogenic plasma extravasation. A similar decrease of I—SP was seen after chronic denervation of the skin. 5 Intra-arterial infusion of substance P (threshold dose 5 × 10-13 mol/min) or physalaemin induced dose-dependent plasma extravasation. Somatostatin, vasoactive intestinal polypeptide, caerulein and the enkephalin-analogue FK 33-824 were ineffective in doses 100 fold higher. 6 The results indicate that the action of capsaicin on substance P neurones is restricted to primary sensory neurones. Since in every case a decreased substance P content of the skin was associated with impaired neurogenic plasma extravasation, it is suggested that release of substance P is involved in neurogenic plasma extravasation.