Antibody and cytokine responses to Giardia excretory/secretory proteins in Giardia intestinalis-infected BALB/c mice
Antibody and cytokine responses to Giardia excretory/secretory proteins in Giardia intestinalis-infected BALB/c mice
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DOI：
10.1007/s00436-014-3927-4
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发表时间：
2014-07-01
影响因子：
2
通讯作者：
Dei-Cas, Eduardo
中科院分区：
文献类型：
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作者：
Jimenez, Juan C.;Fontaine, Josette;Dei-Cas, Eduardo
在实验过程中，观察了对贾第鞭毛虫排泄分泌蛋白和可溶性提取物的体液和细胞反应。BALB/c小鼠的大肠杆菌感染。感染后，检测针对排泄/分泌蛋白和可溶性提取物的IgG 1、IgG 2a、伊加和IgE抗体的产生。好吧用ELISA法检测感染小鼠肠道E/S蛋白和可溶性提取物的特异性伊加抗体。免疫印迹法分别鉴定了伊加和IgG的30、58、63和83 kDa蛋白。免疫后21天脾细胞体外增殖率和白细胞介素-4（IL-4）分泌增加。在感染的BALB/c小鼠中观察到分泌/分泌的蛋白刺激后，在可溶性提取物存在下的低增殖反应。在减少囊肿排出量时（感染后14-21天），干扰素γ（IFN-γ）和白细胞介素-5（IL-5）的高产量结果表明，这些细胞因子在控制感染中起着重要作用。有趣的是，从感染后7天到28天，记录了用两种制剂刺激后白细胞介素-10的进行性和逐渐增加，表明这些抗原对贾第虫感染期间的免疫应答可能具有调节作用。因此，贾第鞭毛虫感染刺激混合应答Th 1和Th 2，主要由排泄/分泌抗原刺激。这些抗原的免疫原性可作为鉴定G.阿里斯
The humoral and cellular responses against excretory/secretory proteins and soluble extracts of Giardia intestinalis were evaluated in the course of experimental G. intestinalis infection in BALB/c mice. Production of IgG1, IgG2a, IgA, and IgE antibodies against excreted/secreted proteins and soluble extract was detected after infection by G. intestinalis. Specific IgA antibody against E/S proteins and soluble extract form intestinal fluids in infected mice was detected by ELISA. The Western blotting identified proteins of 30, 58, 63, and 83 kDa for IgA and IgG, respectively. High proliferation rate in vitro of spleen cell and secretion of interleukin-4 (IL-4) at 21 days p.i. after stimulation with excreted/secreted proteins and low proliferative response in the presence of soluble extract in infected BALB/c mice was observed. High production of interferon gamma (IFN-gamma) and interleukin-5 (IL-5) at the time of decreasing cyst output (14-21 days p.i.) in infected mice was recorded, suggesting the important role of these cytokines in the control of the infection. Interestingly, progressive and gradual increase of the interleukin-10 after stimulation with both preparations was recorded from 7 days until 28 days after infection, indicating the possible regulatory effect of these antigens on the immune response during Giardia infection. Therefore, the infection by Giardia duodenalis stimulates a mixed response Th1 and Th2, mainly stimulated by excretory/secretory antigens. The immunogenicity of these antigens may be a suitable for identification of the proteins related with the effective immune response in the course of infection by G. duodenalsis.