Beneficial rhizobacteria Pseudomonas simiae WCS417 induce major transcriptional changes in plant sugar transport
Beneficial rhizobacteria Pseudomonas simiae WCS417 induce major transcriptional changes in plant sugar transport
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DOI：
10.1093/jxb/eraa396
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发表时间：
2020-12-31
影响因子：
6.9
通讯作者：
Vriet, Cecile
中科院分区：
文献类型：
--
作者：
Desrut, Antoine;Moumen, Bouziane;Vriet, Cecile
植物与复杂的微生物群体密切相关，包括通常称为植物促生长根际细菌（PGPR）的根际细菌物种。PGPR能够提高植物生产力，但这一过程所涉及的分子机制在很大程度上仍然未知。使用在体外实验系统，模式植物拟南芥，以及其特点的PGPR菌株假单胞菌猿猴WCS417 r（PsWCS417 r），我们进行了一系列全面的表型和基因表达分析。我们的研究结果表明，PsWCS417r诱导糖运输和其他关键的生物学过程中的主要转录变化与植物生长，发育和防御。值得注意的是，我们确定了一组13个基因的SWEET和ERD6样糖转运蛋白基因家族，其表达上调或下调幼苗根接种PGPR或暴露于其挥发性化合物。使用反向遗传的方法，我们表明，SWEET11和SWEET12的功能参与的相互作用和其植物生长促进作用，可能通过控制糖的量从地上运输到根和PGPR。总之，我们的研究表明，PGPR诱导的植物生长和发育的有益影响与植物糖运输的变化。
Plants live in close relationships with complex populations of microorganisms, including rhizobacterial species commonly referred to as plant growth-promoting rhizobacteria (PGPR). PGPR are able to improve plant productivity, but the molecular mechanisms involved in this process remain largely unknown. Using an in vitro experimental system, the model plant Arabidopsis thaliana, and the well-characterized PGPR strain Pseudomonas simiae WCS417r (PsWCS417r), we carried out a comprehensive set of phenotypic and gene expression analyses. Our results show that PsWCS417r induces major transcriptional changes in sugar transport and in other key biological processes linked to plant growth, development, and defense. Notably, we identified a set of 13 genes of the SWEET and ERD6-like sugar transporter gene families whose expression is up- or down-regulated in response to seedling root inoculation with the PGPR or exposure to their volatile compounds. Using a reverse genetic approach, we demonstrate that SWEET11 and SWEET12 are functionally involved in the interaction and its plant growth-promoting effects, possibly by controlling the amount of sugar transported from the shoot to the root and to the PGPR. Altogether, our study reveals that PGPR-induced beneficial effects on plant growth and development are associated with changes in plant sugar transport.