LIQUID SCINTILLATION-COUNTING OF AQUEOUS SAMPLES USING TRITON-CONTAINING SCINTILLANTS
LIQUID SCINTILLATION-COUNTING OF AQUEOUS SAMPLES USING TRITON-CONTAINING SCINTILLANTS
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DOI：
10.1016/0003-2697(76)90306-7
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发表时间：
1976-01-01
影响因子：
2.9
通讯作者：
PANDE, SV
中科院分区：
文献类型：
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作者：
PANDE, SV
用甲苯-曲拉通(2：1，VOL/VOL)闪烁体计数水溶性放射性化合物时，由于相分离，在-lt；5%(VOL/VOL)水存在时，计数速率明显不稳定。在这些情况下，不能使用3H2O作为内标进行效率校正，因为在相同条件下，用氚水进行计数的比率是稳定的。将水增加到.gtoreq。6%的人计数率稳定。使用甲苯-Triton(2：1，体积/体积)闪烁剂，14C和~3H标记化合物的水位最好保持在6-12%或18-24%之间，以进行6度计数。C或在环境温度下(但室温下的~3H计数仅在6-12%之间)。用改良的Tritosol含有35毫升乙二醇、140毫升乙醇、250毫升Triton X-100、575毫升二甲苯、3克PPO[2，5-二苯基恶唑]和+-。200 mg的POPOP[(1，4-双-2-[5-苯基恶唑基])-苯]，在室温或6℃时，14C和~3H的水位最高可达23%。C.在这些限制范围内，以甲苯-曲拉通或改性的曲拉通为闪光剂，极性和非极性放射性化合物都表现出相似的效率，并给出了基于外标比的猝灭校正曲线，对于14C和~3H标记的化合物都是线性的。
Markedly unstable count rates were observed using a toluene-Triton (2:1, vol/vol) scintillant during counting of water-soluble radioactive compounds when < 5% (vol/vol) water was present, because of the separation of phases. Efficiency correction in these instances could not be made using 3H2O as internal standard, because under the same conditions count rates with tritiated water were stable. Increasing water to .gtoreq. 6% stabilized the count rates. With toluene-Triton (2:1, vol/vol) scintillant, the water level should preferably be maintained between either 6-12 or 18-24% for 14C- and 3H-labeled compounds for counting at 6.degree. C or at ambient temperature (but only between 6-12% for 3H counting at room temperature). With a Tritosol modified to contain 35 ml of ethylene glycol, 140 ml of ethanol, 250 ml of Triton X-100, 575 ml of xylene, 3 g of PPO [2,5 diphenyloxazole] and .+-. 200 mg of POPOP [(1,4-bis-2-[5-phenyloxazolyl])-benzene], water levels of up to 23% were acceptable for 14C and 3H for counting at room temperature or at 6.degree. C. Within these limitations, with the toluene-Triton or with the modified Tritosol as scintillant, both polar and apolar radioactive compounds exhibited similar efficiencies and gave quench-correction curves, based on the external standard ratio, that were linear for both 14C and 3H-labeled compounds.