Short Communication Characterization of Cestrum yellow leaf curling virus: a new member of the family Caulimoviridae
Short Communication Characterization of Cestrum yellow leaf curling virus: a new member of the family Caulimoviridae
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发表时间：
2003
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通讯作者：
L. Stavolone;A. Ragozzino;T. Hohn
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作者：
L. Stavolone;A. Ragozzino;T. Hohn
蝙蝠葛黄叶卷曲病毒（CmYLCV）是蝙蝠葛花叶病的病原。克隆了该病毒的基因组，并证明该克隆对C. parqui。CmYLCV在感染病毒的植物组织中存在典型的病毒颗粒，其全基因组序列的测定结果表明，CmYLCV属于花椰菜花叶病毒科（Caulimoviridae）的一员，CmYLCV具有植物副反转录病毒的所有特征保守结构域。其基因组全长8253 bp，包含7个开放阅读框（ORF）。系统发育分析的关系与其他成员的花椰菜花叶病毒科揭示，CmYLCV是密切相关的大豆褪绿斑驳病毒（SbCMV）的样属，特别是SbCMV。然而，与该属的其他成员相比，引物结合位点位于ORF Ib之后的顺反子间区域中，而不是在该ORF内，并且对应于ORF VII的ORF缺失。
Cestrum yellow leaf curling virus (CmYLCV) has been characterized as the aetiological agent of the Cestrum parqui mosaic disease. The virus genome was cloned and the clone was proven to be infectious to C. parqui. The presence of typical viroplasms in virus-infected plant tissue and the information obtained from the complete genomic sequence confirmed CmYLCV as a member oftheCaulimoviridaefamily.All characteristic domains conserved inplantpararetroviruses were found in CmYLCV. Its genome is 8253 bp long and contains seven open reading frames (ORFs). Phylogenetic analysis of the relationships with other members of the Caulimoviridae revealed that CmYLCV is closely related to the Soybean chlorotic mottle virus (SbCMV)-like genus and particularly to SbCMV. However, in contrast to the other members of this genus, the primer-binding site is located in the intercistronic region following ORF Ib rather than within this ORF, and an ORF corresponding to ORF VII is missing.