THE THEORY AND APPLICATION OF A NEW METHOD OF DETECTING CHROMOSOMAL REARRANGEMENTS IN DROSOPHILA-MELANOGASTER
THE THEORY AND APPLICATION OF A NEW METHOD OF DETECTING CHROMOSOMAL REARRANGEMENTS IN DROSOPHILA-MELANOGASTER
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DOI：
10.1086/281833
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发表时间：
1954-01-01
影响因子：
2.9
通讯作者：
LEWIS, EB
中科院分区：
文献类型：
--
作者：
LEWIS, EB
一种称为“转移效应”的新型位置效应允许在诱导治疗后的第一代中快速高效地检测到染色体重排。涉及到几个独特的特征：(1)位置效应延伸到比迄今所证明的更远的距离(超过500个唾液腺染色体盘)；(2)有效地检测到全等色以及等色-异色重排；以及(3)位置效应仅在假等位突变基因之间的双重杂合子中检测到，其排列必须是反式的(a+/+b)。结构杂合性对体细胞配对的干扰被认为是为了减少必需基因产物从这个杂合子的一个染色体到另一个染色体的运输。利用这一新方法，已发现快(堆)中子在果蝇体内产生重排时比X射线或伽马射线更有效，并对核爆期间不同站点的快中子剂量进行了估算。
A new type of position effect called the "trans-vection effect" permits rapid and highly efficient detection of chromosomal rearrangements in the first generation following an induction treatment. Several unique features are involved: (1) the position effect extends over vastly greater distances than heretofore demonstrated (over 500 discs of the salivary gland chromosomes); (2) wholly euchromatic as well as euchromatic-heterochromatic rearrangements are efficiently detected; and (3) the position effect is detectable only in a double heterozygote between pseudoallelic mutant genes, the arrangement of which must be of the trans-type (a +/+ b). Interference in somatic pairing exerted by structural heterozygosity is postulated to reduce the transport of an essential gene product from one chromosome of this heterozygote to the other. By the use of this new method fast (pile) neutrons have been found to be more effective than X-rays or gamma rays in producing rearrangements in Drosophila, and estimates of the dose of fast neutrons at different stations during a nuclear detonation have been derived.