An alkaline lipase from organic solvent tolerant Acinetobacter sp EH28: Application for ethyl caprylate synthesis
An alkaline lipase from organic solvent tolerant Acinetobacter sp EH28: Application for ethyl caprylate synthesis
复制标题
DOI：
10.1016/j.biortech.2009.12.107
复制
发表时间：
2010-05-01
影响因子：
11.4
通讯作者：
Madamwar, Datta
中科院分区：
文献类型：
--
作者：
Ahmed, Eltayib Hassan;Raghavendra, Tripti;Madamwar, Datta
从富油土壤中分离到一株产耐高温碱性脂肪酶的中温细菌，经鉴定为不动杆菌属。EH28。采用硫酸铵沉淀和疏水作用层析的方法对该脂肪酶进行部分纯化，纯化倍数为24.2倍，比活力为57.1 U/ml。部分纯化的酶在pH 10.0和50℃时活性最高，在50℃下高度稳定，在90分钟内仍保持100%的活性。它非常稳定，在各种有机溶剂中保持了80%以上的初始活性。利用EH28脂肪酶在有机溶剂中合成香料辛酸乙酯，为不动杆菌属的应用提供了一个概念。脂肪酶在非水催化中的应用。最适合该酯化反应的反应条件为：反应温度40℃，辛酸与乙醇的摩尔比为13：1，反应介质为环己烷。(C)2009爱思唯尔有限公司。保留所有权利。
A mesophilic bacterium producing a thermostable alkaline lipase was isolated from oil rich soil sample and identified as Acinetobacter sp. EH28. The lipase was partially purified by ammonium sulphate precipitation followed by hydrophobic interaction chromatography with 24.2-fold purification and 57.1 U/ml specific activity. The partially purified enzyme exhibited maximum activity at pH 10.0 and at 50 degrees C and was highly stable at 50 degrees C retaining 100% of its activity up to 90 min. It was highly stable and retained more than 80% of its initial activity upon exposure to various organic solvents. The EH28 lipase was used for synthesis of the flavor ester ethyl caprylate in organic solvents, thus providing a concept of application of Acinetobacter sp. lipase in non-aqueous catalysis. Reaction parameters best suited for this esterification reaction were 40 degrees C reaction temperature, 13:1 ratio of caprylic acid to ethanol and cyclohexane as the medium. (c) 2009 Elsevier Ltd. All rights reserved.