Effects of different abiotic stresses on carotenoid and fatty acid metabolism in the green microalgaDunaliella salinaY6
Effects of different abiotic stresses on carotenoid and fatty acid metabolism in the green microalgaDunaliella salinaY6
复制标题
不同非生物胁迫对绿色微藻Dunaliella salinaY6类胡萝卜素和脂肪酸代谢的影响
DOI：
10.1186/s13213-020-01588-3
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发表时间：
2020
影响因子：
3
通讯作者：
Wang Jiangxin
中科院分区：
文献类型：
--
作者：
Wu Mingcan;Zhu Rongfang;Lu Jiayang;Lei Anping;Zhu Hui;Hu Zhangli;Wang Jiangxin
目的在不同的非生物胁迫条件下，单细胞绿微藻褐藻积累了大量的类胡萝卜素，并伴有脂肪酸的生物合成。类胡萝卜素和脂肪酸都具有长碳骨架；然而，类胡萝卜素和脂肪酸代谢之间的关系是有争议的，并且在微藻中仍然知之甚少。方法采用生长曲线和β -胡萝卜素、叶黄素、脂质、脂肪酸含量测定。salinaY6生长在不同的非生物胁迫条件下，包括强光、缺氮和高盐度。结果高盐度和缺氮条件均能显著抑制细胞生长。缺氮显著诱导β -胡萝卜素积累，而强光促进叶黄素的产生。在三种测试的非生物胁迫条件下，脂质积累与β -胡萝卜素和叶黄素积累没有直接的正相关关系，并且在特定条件下只有少数脂肪酸水平增加。结论在不同的非生物胁迫条件下，细胞β -胡萝卜素积累与特定脂肪酸（C16:0、C18:3n3、C14:0和C15:0）的积累呈正相关，而与总脂肪酸含量呈正相关。
Purpose Under different abiotic-stress conditions, the unicellular green microalgaDunaliella salinaaccumulates large amounts of carotenoids which are accompanied by fatty acid biosynthesis. Carotenoids and fatty acids both possess long carbon backbones; however, the relationship between carotenoid and fatty acid metabolism is controversial and remains poorly understood in microalgae. Methods In this study, we investigated the growth curves and the beta-carotene, lutein, lipid, and fatty acid contents ofD. salinaY6 grown under different abiotic-stress conditions, including high light, nitrogen depletion, and high salinity. Results Both high-salinity and nitrogen-depleted conditions significantly inhibited cell growth. Nitrogen depletion significantly induced beta-carotene accumulation, whereas lutein production was promoted by high light. The accumulation of lipids did not directly positive correlate with beta-carotene and lutein accumulation under the three tested abiotic-stress conditions, and levels of only a few fatty acids were increased under specific conditions. Conclusion Our data indicate that cellular beta-carotene accumulation inD. salinaY6 positive correlates with accumulation of specific fatty acids (C16:0, C18:3n3, C14:0, and C15:0) rather than with total fatty acid content under different abiotic stress conditions.