A Novel Shell-Structure Cell Microcarrier (SSCM) for Cell Transplantation and Bone Regeneration Medicine
A Novel Shell-Structure Cell Microcarrier (SSCM) for Cell Transplantation and Bone Regeneration Medicine
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DOI：
10.1007/s11095-010-0321-5
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发表时间：
2011-06-01
影响因子：
3.7
通讯作者：
Wang, Dong-An
中科院分区：
文献类型：
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作者：
Su, Kai;Gong, Yihong;Wang, Dong-An
本研究旨在开发一种新型的开放空心壳结构细胞微载体（SSCM），以提高锚定依赖性细胞（ADC）的装载效率，增加细胞增殖和组织再生的空间，更好地提高其治疗效果。方法采用油/水/油（o/w/o）法制备明胶颗粒，采用可调表面交联技术对明胶颗粒进行改性，随后释放非交联材料。利用光学显微镜和扫描电镜（SEM）观察了微载体的形貌。通过细胞负载试验评价SSCM的生物相容性及对成骨的影响。结果采用表面法制备了SSCMs。这种壳状结构可以使细胞在球的内外表面附着生长，为细胞增殖和细胞外基质（ECM）的分泌提供充足的空间。细胞载量、增殖率和成骨相关基因表达量均高于球形明胶微载体。结论sscm具有良好的显微结构、良好的细胞相容性和较强的细胞亲和性，是治疗性细胞移植到组织再生范围的良好载体。
Purpose The present study aims to develop a novel open and hollow shell-structure cell microcarrier (SSCM) to improve the anchorage-dependent cell (ADC) loading efficiency, increase the space for cell proliferation and tissue regeneration, and better propel its therapeutic effects.Methods Gelatin particles were prepared with oil/water/oil (o/w/o) technique and modified by an adjustable surface crosslinking technique and subsequent release of uncrosslinked material. Optical microscopy and scanning electron microscopy (SEM) were utilized to observe the morphologies of the microcarriers. Cell loading tests were performed to evaluate the biocompatibilities and effect on osteogenesis of SSCM.Results SSCMs were successfully fabricated via the surface technique. The shell-structure could allow the cell to attach and grow on both outer and inner surface of sphere and provide adequate space for cell proliferation and extracellular matrix (ECM) secretion. The cell loading rate, proliferation rate and osteogenesis-related gene expressions on the SSCMs were higher than those on the spherical gelatin microcarriers.Conclusions The outstanding performance of injectable SSCMs endowed with favorable micro-structure, desirable cytocompatibility and enhanced cell affinity makes them as a good choice as cell delivery vehicle for transplanting therapeutic cells towards the scope of tissue regeneration.