Cytochrome P450sCYP380C6andCYP380C9in green peach aphid facilitate its adaptation to indole glucosinolate-mediated plant defense
Cytochrome P450sCYP380C6andCYP380C9in green peach aphid facilitate its adaptation to indole glucosinolate-mediated plant defense
复制标题
绿桃蚜中的细胞色素P450s CYP380C6和CYP380C9促进其适应吲哚芥子油苷介导的植物防御
DOI：
10.1002/ps.6002
复制
发表时间：
2020-08-20
影响因子：
4.1
通讯作者：
Zhu-Salzman, Keyan
中科院分区：
文献类型：
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作者：
Ji, Rui;Lei, Jiaxin;Zhu-Salzman, Keyan
背景在拟南芥中过表达CCA 1-ox增加吲哚硫代葡萄糖苷的产生和对绿色桃蚜（Myzus persicae）的抗性。蚜虫对这组植物防御化合物的反应或潜在的分子机制知之甚少。结果在CCA 1-ox上饲养40代以上的蚜虫（即CCA种群）比在野生型Col-0上饲养的蚜虫（即COL种群）对CCA 1-ox的敏感性降低。这种提高的耐受性是跨代的，因为在CCA群体转移到Col-0后，它保持了至少八代。有趣的是，转录组分析表明，所有差异细胞色素P450单加氧酶基因（MpCYP），主要是MpCYP 4s，MpCYP 380和MpCYP 6s，在CCA群体中更高表达。应用P450抑制剂的CCA人口导致减少蚜虫繁殖上CCA 1-ox，这是没有观察到，如果蚜虫饲养的Col-0。当使用病毒诱导的基因沉默阻断CCA 1-ox中吲哚芥子油苷的生物合成时，P450抑制剂对CCA群体的影响减弱，证实了MpCYP在抵消CCA 1-ox中的防御机制中发挥的重要作用，Col-0中的防御机制较低或不存在。此外，我们使用宿主诱导的基因沉默来鉴定MpCYP 380 C6和MpCYP 380 C9，其特异性地促进CCA群体科普CCA 1介导的植物防御。表达谱揭示了它们可能对蚜虫中观察到的跨代耐受性的贡献。结论蚜虫MpCYP 380 C6和MpCYP 380 C9基因在抑制吲哚芥子油苷介导的植物防御中起重要作用，且这种作用具有跨代性。
BACKGROUND OverexpressingCIRCADIAN CLOCK ASSOCIATED1inArabidopsis thaliana(CCA1-ox) increases indole glucosinolate production and resistance to green peach aphid (Myzus persicae). Little is known of how aphids respond to this group of plant defense compounds or of the underlying molecular mechanism. Results Aphids reared onCCA1-oxfor over 40 generations (namely the CCA population) became less susceptible toCCA1-oxthan aphids maintained on the wild-type Col-0 (namely the COL population). This elevated tolerance was transgenerational as it remained for at least eight generations after the CCA population was transferred to Col-0. Intriguingly, transcriptome analysis indicated that all differential cytochrome P450 monooxygenase genes (MpCYPs), primarilyMpCYP4s,MpCYP380sandMpCYP6s, were more highly expressed in the CCA population. Application of a P450 inhibitor to the CCA population resulted in decreased aphid reproduction onCCA1-ox, which was not observed if aphids were reared on Col-0. When indole glucosinolate biosynthesis inCCA1-oxwas blocked using virus-induced gene silencing, the effect of the P450 inhibitor on the CCA population was attenuated, affirming the essential role played by MpCYPs in counteracting the defense mechanism inCCA1-oxthat is low or absent in Col-0. Furthermore, we used host-induced gene silencing to identify MpCYP380C6 and MpCYP380C9 that specifically facilitated the CCA population to cope withCCA1-mediated plant defense. Expression profiles revealed their possible contribution to the transgenerational tolerance observed in aphids. Conclusion MpCYP380C6 and MpCYP380C9 in aphids play a crucial role in mitigating indole glucosinolate-mediated plant defense, and this effect is transgenerational.