Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture.
Ribavirin Inhibits Parrot Bornavirus 4 Replication in Cell Culture.
复制标题
DOI：
10.1371/journal.pone.0134080
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Tizard IR
中科院分区：
文献类型：
--
作者：
Musser JM;Heatley JJ;Koinis AV;Suchodolski PF;Guo J;Escandon P;Tizard IR
鹦鹉博尔纳病毒4型（Parrot bornavirus 4）是引起腺室扩张病（proventricular dilatation disease）的病原体，腺室扩张病是鹦鹉和其它鸟类的致命性神经和胃肠道疾病。我们测试了利巴韦林（一种对一系列RNA和DNA病毒具有抗病毒活性的抗病毒核苷类似物）抑制鸭胚胎成纤维细胞中鹦鹉博纳病毒4型复制的能力。使用了两种评价不同病毒复制产物的分析方法，即病毒特异性核蛋白的间接免疫细胞化学和病毒特异性磷蛋白基因mRNA的qRT-PCR。在感染的鸭胚胎成纤维细胞中，2.5 - 25 μg/mL的利巴韦林抑制鹦鹉博纳病毒4型复制，降低病毒mRNA和病毒蛋白载量。鸟苷的加入降低了利巴韦林的抗病毒活性，这表明抗鹦鹉博纳病毒4的一种可能的作用机制可能是通过抑制肌苷一磷酸脱氢酶。本研究证明了细胞培养中鹦鹉博尔纳病毒4型对利巴韦林的敏感性。
Parrot bornavirus 4 is an etiological agent of proventricular dilatation disease, a fatal neurologic and gastrointestinal disease of psittacines and other birds. We tested the ability of ribavirin, an antiviral nucleoside analog with antiviral activity against a range of RNA and DNA viruses, to inhibit parrot bornavirus 4 replication in duck embryonic fibroblast cells. Two analytical methods that evaluate different products of viral replication, indirect immunocytochemistry for viral specific nucleoprotein and qRT-PCR for viral specific phosphoprotein gene mRNA, were used. Ribavirin at concentrations between 2.5 and 25 μg/mL inhibited parrot bornavirus 4 replication, decreasing viral mRNA and viral protein load, in infected duck embryonic fibroblast cells. The addition of guanosine diminished the antiviral activity of ribavirin suggesting that one possible mechanism of action against parrot bornavirus 4 may likely be through inosine monophosphate dehydrogenase inhibition. This study demonstrates parrot bornavirus 4 susceptibility to ribavirin in cell culture.