Transfer of a protein pattern from self-assembled DNA origami to a functionalized substrate
Transfer of a protein pattern from self-assembled DNA origami to a functionalized substrate
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DOI：
10.1039/c3cc37408d
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发表时间：
2013-01-01
影响因子：
4.9
通讯作者：
Gothelf, Kurt V.
中科院分区：
文献类型：
--
作者：
Busuttil, Katerina;Rotaru, Alexandru;Gothelf, Kurt V.
DNA折纸上的蛋白质链霉亲和素图案被打印在功能化的表面上。链霉亲和素通过二硫键间隔物连接到DNA上，这些连接物在DNA折纸上自组织成预定的模式。该结构固定在生物素-SAM功能化的金表面上。通过二硫键裂解，折纸从链霉亲和素中分离出来，将蛋白质图案留在表面。
Patterns of the protein streptavidin on DNA origami are printed onto a functionalized surface. The streptavidins are linked to DNA via a disulfide spacer and these conjugates self-organise into a predetermined pattern on DNA origami. The structure immobilises on a biotin-SAM-functionalized Au surface. By disulfide cleavage the origami is separated from the streptavidins, leaving the protein pattern at the surface.