Effect of 3′,5′-cyclic diguanylic acid in a broiler Clostridium perfringens infection model
Effect of 3′,5′-cyclic diguanylic acid in a broiler Clostridium perfringens infection model
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DOI：
10.3382/ps.2013-03143
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发表时间：
2013-10-01
期刊：
影响因子：
4.4
通讯作者：
Diarra, Moussa S.
中科院分区：
文献类型：
--
作者：
Fatima, Mussarat;Rempel, Heidi;Diarra, Moussa S.
为了探索控制产气荚膜梭菌的策略，我们在肉鸡攻击模型中研究了普遍存在的细菌第二信使3 '，5'-环二鸟苷酸（c-di-GMP）与青霉素G的协同效应。在第14、15和16天，所有鸡通过管饲法在作物中接种4C的混合物。产气荚膜杆菌菌株。在第24天通过管饲或肌内（IM）用盐水（对照组）或20 nmol c-di-GMP处理鸡，所有处理均与青霉素G水溶液联合进行5天。在第21 ~ 35天每周采集盲肠和回肠样品。产气荚膜杆菌和乳酸杆菌计数。在35日龄时，IM处理显著降低C（P < 0.05）.在盲肠中的产气荚膜杆菌，提示青霉素G和c-di-GMP对C.肉鸡盲肠产气荚膜杆菌此外，还对盲肠DNA进行了一系列C.产气荚膜梭菌毒力基因显示，IM处理组中从第21天到第35天，产气荚膜梭菌α-毒素基因（cpa）的流行率为30%，而其他2个组（对照组和管饲组）中从第21天到第35天，cpa基因的出现率从10%增加到60%。从第21天至第35天，在相同样本中检测到指示革兰氏阴性菌的β-内酰胺酶基因（bla（CMY-2）、bla（SHV）和bla（TEM）），未显示出显著的治疗效应。扩增片段长度多态性显示，21日龄对照鸡和35日龄IM处理的c-di-GMP组之间的盲肠具有92%的相似性。这表明，c-di-GMP IM处理可能在被C.产气荚膜杆菌我们的研究结果表明，c-di-GMP可以减少C.在不增加对一些β-内酰胺酶基因的选择压力或改变肠道细菌群体的情况下，
In an effort to explore strategies to control Clostridium perfringens, we investigated the synergistic effect of a ubiquitous bacterial second messenger 3',5'-cyclic diguanylic acid (c-di-GMP) with penicillin G in a broiler challenge model. All chicks were inoculated in the crop by gavage on d 14, 15, and 16 with a mixture of 4 C. perfringens strains. Birds were treated with saline (control group) or 20 nmol of c-di-GMP by gavage or intramuscularly (IM) on d 24, all in conjunction with penicillin G in water for 5 d. Weekly samplings of ceca and ileum were performed on d 21 to 35 for C. perfringens and Lactobacillus enumeration. On d 35 of age, the IM treatment significantly (P < 0.05) reduced C. perfringens in the ceca, suggesting possible synergistic activity between penicillin G and c-di-GMP against C. perfringens in broiler ceca. Moreover, analysis of ceca DNA for the presence of a series of C. perfringens virulence genes showed a prevalence of 30% for the Clostridium perfringens alpha-toxin gene (cpa) from d 21 to 35 in the IM-treated group, whereas the occurrence of the cpa gene increased from 10 to 60% in the other 2 groups (control and gavage) from d 21 to 35. Detection of beta-lactamase genes (bla(CMY-2), bla(SHV), and bla(TEM)) indicative of gram-negative bacteria in the same samples from d 21 to 35 did not show significant treatment effects. Amplified fragment-length polymorphism showed a predominant 92% similarity between the ceca of 21-d-old control birds and the 35-d-old IM-treated c-di-GMP group. This suggests that c-di-GMP IM treatment might be effective at restoring the normal microflora of the host on d 35 after being challenged by C. perfringens. Our results suggest that c-di-GMP can reduce the colonization of C. perfringens in the gut without increasing the selection pressure for some beta-lactamase genes or altering the commensal bacterial population.