A late embryogenesis abundant protein GsPM30 interacts with a receptor like cytoplasmic kinase GsCBRLK and regulates environmental stress responses
A late embryogenesis abundant protein GsPM30 interacts with a receptor like cytoplasmic kinase GsCBRLK and regulates environmental stress responses
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胚胎发生晚期丰富的蛋白 GsPM30 与细胞质激酶 GsCBRLK 等受体相互作用并调节环境应激反应
DOI：
10.1016/j.plantsci.2019.02.015
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发表时间：
2019
期刊：
影响因子：
5.2
通讯作者：
Xiaoli Sun
中科院分区：
文献类型：
--
作者：
Mingzhe Sun;Yang Shen;Kuide Yin;Yongxia Guo;Xiaoxi Cai;Junkai Yang;Yanming Zhu;Bowei Jia;Xiaoli Sun
AG 甘氨酸 soja 受体样细胞质激酶 GsCBRLK 以前被描述为耐盐性的正调节因子。然而，GsCBRLK 如何调节应激反应仍不清楚。在这里，我们报告了 GsCBRLK 与第 3 组胚胎发生晚期丰富蛋白 GsPM30 之间的相互作用，并表明其在应激反应中的作用。通过酵母两种杂交测定，发现 GsPM30 与 GsCBRLK 物理结合，并通过双分子荧光互补分析验证。缺失分析表明 GsCBRLK 的 N 端可变结构域足以与 GsPM30 相互作用。除GsPM30外，GsCBRLK还可以与多个第3组LEA相关联，其中N端序列与GsPM30表现出高度同一性。 GsCBRLK 与其他与 GsPM30 关系不太密切的第 3 组 LEA 之间观察到较低的结合亲和力甚至没有相互作用。此外，我们观察到 GsPM30 可以定位于植物细胞的内周以及细胞质和细胞核中。此外，GUS染色和定量实时PCR结果表明GsPM30在不同组织中普遍表达，并通过NaCl和甘露醇处理诱导表达。一致地，GsPM30在拟南芥中的过表达导致幼苗和成苗阶段对高盐度和脱水/缺水的耐受性增加。我们的结果证明了 GsCBRLK 和 LEA 之间的相互作用，并揭示了 GsPM30 在应激反应中的积极作用。
AGlycine sojareceptor like cytoplasmic kinase GsCBRLK was previously characterized as a positive regulator of salt tolerance. However, how GsCBRLK regulates stress responses remains obscure. Here, we report the interaction between GsCBRLK and a group 3 late embryogenesis abundant protein GsPM30, and suggest its role in stress responses. GsPM30 was found to physically associate with GsCBRLK through yeast two hybrid assays, which was verified by bimolecular fluorescence complementation analysis. Deletion analyses showed that the N-terminal variable domain of GsCBRLK was sufficient for GsPM30 interaction. Besides GsPM30, GsCBRLK could associate with several group 3 LEAs, of which the N-terminus sequences show high identity with GsPM30. Lower binding affinity or even no interaction was observed between GsCBRLK and other group 3 LEAs, which are less closely related to GsPM30. Furthermore, we observed that GsPM30 could localize surrounding the internal circumference of plant cells, as well as in cytoplasm and nucleus. In addition, GUS staining and quantitative real-time PCR results suggested the ubiquitous expression in different tissues and induced expression by NaCl and mannitol treatments forGsPM30. Consistently,GsPM30overexpression inArabidopsiscaused increased tolerance to high salinity and dehydration/water deficit at both the young and adult seedling stages. Our results demonstrated the interaction between GsCBRLK and LEAs, and revealed the positive role ofGsPM30in stress responses.