CmWRKY1 Enhances the Dehydration Tolerance of Chrysanthemum through the Regulation of ABA-Associated Genes.
CmWRKY1 Enhances the Dehydration Tolerance of Chrysanthemum through the Regulation of ABA-Associated Genes.
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CmWRKY1通过ABA相关基因的调控增强菊花的脱水耐受性
DOI：
10.1371/journal.pone.0150572
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Chen F
中科院分区：
文献类型：
--
作者：
Fan Q;Song A;Jiang J;Zhang T;Sun H;Wang Y;Chen S;Chen F
WRKY 转录因子在植物的各种非生物胁迫反应中充当拮抗或协同调节剂。在这里，我们发现 CmWRKY1 是从菊花中分离出来的 IIb WRKY 家族的成员，在酵母细胞中没有表现出转录激活。亚细胞定位检查表明CmWRKY1在体内定位于细胞核。此外，与野生型植物相比，CmWRKY1 过表达转基因株系对聚乙二醇 (PEG) 处理的响应表现出增强的脱水耐受性。我们进一步证实，转基因植物表现出ABA负调控基因（如PP2C、ABI1和ABI2）的表达水平受到抑制，而ABA正调控基因（如PYL2、SnRK2.2、ABF4、MYB2、RAB18和DREB1A）的表达水平被激活。综上所述，我们的结果表明，CmWRKY1 通过 ABA 介导的途径在菊花应对干旱的过程中发挥着重要作用。
WRKY transcription factors serve as antagonistic or synergistic regulators in a variety of abiotic stress responses in plants. Here, we show that CmWRKY1, a member of the group IIb WRKY family isolated from Chrysanthemum morifolium, exhibits no transcriptional activation in yeast cells. The subcellular localization examination showed that CmWRKY1 localizes to the nucleus in vivo. Furthermore, CmWRKY1-overexpressing transgenic lines exhibit enhanced dehydration tolerance in response to polyethylene glycol (PEG) treatment compared with wild-type plants. We further confirmed that the transgenic plants exhibit suppressed expression levels of genes negatively regulated by ABA, such as PP2C, ABI1 and ABI2, and activated expression levels of genes positively regulated by ABA, such as PYL2, SnRK2.2, ABF4, MYB2, RAB18, and DREB1A. Taken together, our results indicate that CmWRKY1 plays an important role in the response to drought in chrysanthemum through an ABA-mediated pathway.