Prevalence of calf diarrhea caused by bovine group A rotavirus carrying G serotype 8 specificity

Prevalence of calf diarrhea caused by bovine group A rotavirus carrying G serotype 8 specificity
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DOI:
10.1016/s0378-1135(99)00021-8
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发表时间:
1999-05-01
影响因子:
3.3
通讯作者:
Itou, Y
Itou, Y
中科院分区:
农林科学2区
文献类型:
--
作者:
Fukai, K;Sakai, T;Itou, Y

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1995年和1996年从日本鹿儿岛县腹泻犊牛中采集了117份直肠粪便标本。对117份牛A组轮状病毒进行了酶免疫检测,其中43份为阳性,33份为分离阳性。用逆转录-聚合酶链反应对33株分离株进行G血清型、P血清型和P基因型鉴定,其中G血清8型20株(60.6%)。因此,我们发现,牛组轮状病毒携带G血清型8特异性引起的犊牛腹泻在本研究期间在该地区流行。据我们所知,这是第一份关于牛A组轮状病毒携带G血清8型特异性引起犊牛腹泻的报告。(C)1999 Elsevier Science B. V.保留所有权利。
One hundred and seventeen rectal fecal specimens were collected in 1995 and 1996 from calves with diarrhea in Kagoshima Prefecture in Japan. The bovine group A rotavirus was detected by enzyme immunoassay in 43 of 117 specimens and isolated from 33 of the 43 specimens that were positive. G serotype, P serotype, and P genotype of 33 isolates were identified by reverse transcription-polymerase chain reaction, and 20 of 33 isolates (60.6%) were identified as G serotype 8. Thus, we discovered that calf diarrhea caused by bovine group A rotavirus carrying G serotype 8 specificity was prevalent in this research area during this research period. To our knowledge, this is the first report on the prevalence of calf diarrhea caused by the bovine group A rotavirus carrying G serotype 8 specificity. (C) 1999 Elsevier Science B.V. All rights reserved.