Introgression of Yield Component Traits in Rice (Oryza sativa ssp indica) through Interspecific Hybridization
Introgression of Yield Component Traits in Rice (Oryza sativa ssp indica) through Interspecific Hybridization
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DOI：
10.2135/cropsci2015.11.0693
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发表时间：
2017-05-01
期刊：
影响因子：
2.3
通讯作者：
Singh, Kuldeep
中科院分区：
文献类型：
--
作者：
Bhatia, Dharminder;Joshi, Shailesh;Singh, Kuldeep
考虑到野生稻的潜力，以及需要提高水稻的产量潜力和增加水稻产量以满足日益增长的需求，通过杂交六个“AA”基因组物种的70个加入物产生了1780个回交自交系（BIL）：OryzaglaberrimaSteud.，巴氏野生稻切弗，普通野生稻（Oryza rufipogon Griff.）长雄稻切弗& Roehr.，和Oryza glumaepatula Steud.以两个优良品种为试验材料。根据DNA标记和农艺性状的变异选择野生种。BILs的产生是通过将F（1）与受体亲本回交两次（某些情况下为三次），然后连续自交并选择产量和产量构成性状。在3年的增强设计中对1780件BC2F5至BC2F10 BIL进行了评价。采用随机完全区组设计，对108个农艺性状优良的上级生物学指标进行方差分析。在108个BIL中，有15个BIL的产量优势大于轮回亲本10%，其中每穗颖花数和千粒重是影响产量的最显著性状。根据供体材料对产量构成性状改良的贡献，对6份O。glaberrima，O. barthii，O. nivara，O. rufipogon和O. longistaminata和O. glumaepatula被确定为潜在的供体，可以更严格地用于将基因转移到栽培水稻。在本研究中产生的BILs代表了一个新的遗传变异的水库。
Considering the potentials of wild rice (Oryza spp.), as well as the need to enhance the yield potential of rice and to increase rice production to meet increasing demands, 1780 backcross inbred lines (BILs) were generated by crossing 70 accessions of six "AA" genome species: Oryza glaberrima Steud., Oryza barthii A. Chev., Oryza nivara Sharma & Shastry, Oryza rufipogon Griff., Oryza longistaminata A. Chev. & Roehr., and Oryza glumaepatula Steud. with two elite cultivars of Oryza sativa L. The wild species accessions were selected based on variation at the DNA markers and agromorphological traits. The BILs were generated by backcrossing F(1)s twice (thrice in some cases) to recipient parents, followed by continuous selfing and selection for the yield and yield component traits. The set of 1780 BC2F5 to BC2F10 BILs were evaluated in an augmented design over 3 yr. Based on ANOVA in augmented design, 108 agronomically superior BILs were selected and evaluated in randomized complete block design. Of the 108 BILs evaluated, 15 BILs showed >10% yield superiority over the recurrent parent, with spikelet per panicle and thousand-grain weight as most significant traits contributing to yield increase. Based on the contribution of donor accessions for the improvement of yield component traits, six accessions of O. glaberrima, one of O. barthii, two of O. nivara, two of O. rufipogon, and one each of O. longistaminata and O. glumaepatula were identified as potential donors that could be used more rigorously in transferring genes to cultivated rice. The BILs generated in the present study represents a reservoir of novel genetic variation.