Does sperm DNA fragmentation correlate with semen parameters?
Does sperm DNA fragmentation correlate with semen parameters?
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DOI：
10.1002/rmb2.12297
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发表时间：
2019-09-03
影响因子：
3.4
通讯作者：
Ngoc Thanh Cao
中科院分区：
文献类型：
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作者：
Minh Tam Le;Tam An Thi Nguyen;Ngoc Thanh Cao
目的探讨精液常规分析评价的精子质量与精子DNA完整性测定的关系。方法在我们的横断面研究中，从2017年12月至2019年3月在越南顺化生殖内分泌与不孕症中心招募了来自不育夫妇的318名男性。检测一般特征和精液参数。采用精子染色质分散（SCD）测定法测定精子DNA碎片化指数（DFI）。采用DFI 30%阈值对正常组（DFI < 30%）和异常组（DFI >= 30%）进行分类。采用Spearman秩相关系数分析DFI与精液参数的相关性。结果在相关分析中，DFI与头颅异常、进行性运动性呈显著相关，与头颅异常呈正相关（rho = 0.202, P = 0.0003），与进行性运动性呈弱负相关（rho = - 0.168, P = 0.0027）。在双变量分析中，DFI与男性年龄、吸烟和饮酒相关，P < 0.05。结论精子DFI与常规精液参数相关性不强。因此，精子DNA片段分析应作为男性生育能力调查的附加步骤。
Purpose This study aimed to investigate the association between sperm quality assessed by routine semen analysis and sperm DNA integrity assay. Methods In our cross-sectional study, a total of 318 men from the infertile couples were enrolled from December 2017 to March 2019 at the Hue Center for Reproductive Endocrinology and Infertility, Vietnam. General characteristics and semen parameters were detected. The sperm DNA fragmentation index (DFI) was estimated by the sperm chromatin dispersion (SCD) assay. A threshold of DFI 30% was applied to classify normal (DFI < 30%) or abnormal (DFI >= 30%) groups. The correlations between DFI and semen parameters were analyzed by Spearman's rank correlation coefficient. Results In the correlation analysis, DFI was significantly correlated with abnormal head and progressive motility, with a positive correlation with abnormal head (rho = .202, P = .0003) and a weak negative correlation with progressive motility (rho = -.168, P = .0027), respectively. In the bivariate analysis, DFI was associated with male age, smoking, and alcohol consumption with P < .05. Conclusions The sperm DFI was not strongly correlated with conventional semen parameters. Therefore, a sperm DNA fragmentation assay should be performed as an additional step in the investigation of male fertility.