Identification and cholesterol quantification of low density lipoprotein subclasses in young adults by VAP-II methodology.
Identification and cholesterol quantification of low density lipoprotein subclasses in young adults by VAP-II methodology.
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发表时间：
1995-11
影响因子：
6.5
通讯作者：
Krishnaji R Kulkarni;David W. Garber;Martin K. Jones;J. Segrest
中科院分区：
文献类型：
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作者：
Krishnaji R Kulkarni;David W. Garber;Martin K. Jones;J. Segrest
低密度脂蛋白（LDL）颗粒在大小、密度和化学组成上是不均匀的;小而致密的LDL可能比大而漂浮的LDL更易致动脉粥样硬化。我们已经开发了一种快速的微量方法称为LDL VAP-II（垂直自动轮廓-II），用于定量LDL亚类中的胆固醇。该方法是基于一个短（1小时）单垂直自旋密度梯度超离心和在线VAP-II分析仪。LDL VAP-II快速且可重复。使用这种方法，在由195名医学生（年龄，22-29岁）组成的人群中确定了5个LDL亚类，指定为LDL-1（最具浮力）至LDL-5（最密集）。的Rf（密度梯度中LDL主峰的相对位置; Rf值越高，峰值密度越低）与高密度脂蛋白（HDL）胆固醇水平呈显著正相关（r = 0.594），HDL3（0.350）和HDL 2（0.625），与甘油三酯（TG）呈显著负相关极低密度脂蛋白（VLDL）（-0.386）和中密度脂蛋白（IDL）（-0.432）的胆固醇水平。这些结果与其他研究者的结果一致。Rf值与非变性梯度凝胶电泳测定的峰粒径显著相关（r = 0.859）。在比较Rf与性别、VLDL、LDL、Lp[a]、IDL、HDL 3、HDL 2和甘油三酯的前向逐步多变量分析中，仅HDL 2保留在模型中。
Low density lipoprotein (LDL) particles are heterogeneous in size, density, and chemical composition; small, dense LDL may be more atherogenic than large, buoyant LDL. We have developed a rapid microscale method called LDL VAP-II (Vertical Auto Profile-II) for quantification of cholesterol in LDL subclasses. The method is based upon a short (1 h) single vertical spin density-gradient ultracentrifugation and on-line VAP-II analyzer. LDL VAP-II is rapid and reproducible. Using this method five LDL subclasses, designated as LDL-1 (most buoyant) through LDL-5 (most dense), have been identified in a population consisting of 195 medical students (ages, 22-29 years). The Rf (relative position of the major LDL peak in the density gradient; the higher the Rf value, the lower the peak density) was significantly positively correlated with cholesterol levels of high density lipoprotein (HDL) (r = 0.594), HDL3 (0.350) and HDL2 (0.625), and significantly negatively correlated with triglycerides (TG) (-0.355) and cholesterol levels of very low density lipoprotein (VLDL) (-0.386) and intermediate density lipoprotein (IDL) (-0.432). These results are consistent with those obtained by other investigators. The Rf value was significantly correlated with peak particle diameter as determined by non-denaturing gradient gel electrophoresis (r = 0.859). In a forward stepwise multivariate analysis comparing Rf with sex, VLDL, LDL, Lp[a], IDL, HDL3, HDL2, and triglyceride, only HDL2 remained in the model.