IDENTIFICATION OF A 2ND TRANSFORMING REGION IN BOVINE PAPILLOMAVIRUS DNA
IDENTIFICATION OF A 2ND TRANSFORMING REGION IN BOVINE PAPILLOMAVIRUS DNA
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DOI：
10.1073/pnas.81.24.7880
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
LOWY, DR
中科院分区：
文献类型：
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作者：
SCHILLER, JT;VASS, WC;LOWY, DR
牛乳头瘤病毒1型（BPV-1）被用作研究乳头瘤病毒遗传学的模型，因为BPV-1病毒粒子或BPV-1基因组病毒DNA能有效地诱导某些培养细胞的形态转化。先前对bpv -1诱导转化的研究发现，克隆的基因组5.4千碱基（kb）片段（69T）正在转化，如果被逆转录病毒调控元件（长末端重复）激活，该片段3”端的2.3 kb片段也在转化。69T在其5”端附近包含另一个转换段，该转换段也可以通过长末端重复激活。由于第2段与3‘’转化段不重叠，因此BPV-1至少编码2个可以独立转化培养细胞的基因。对5”转化段的突变分析表明，该段的转化基因位于E6开放阅读框内。2个转化片段的生物活性不同，含e6的片段能转化C127小鼠细胞，而不能转化NIH3T3小鼠细胞；3”片段可以转化两种细胞系。
Bovine papillomavirus type 1 (BPV-1) was used as a model for studying papillomavirus genetics because BPV-1 virions or BPV-1 genomic viral DNA efficiently induce morphologic transformation of certain cultured cells. Previous studies of BPV-1-induced transformation have found that a cloned 5.4-kilobase (kb) fragment (69T) of the genome is transforming and that a 2.3-kb segment from the 3'' end of this fragment is also transforming if activated by a retroviral regulatory element (the long terminal repeat). 69T contains another transforming segment near its 5'' end that can also be activated by a long terminal repeat. Since this 2nd segment does not overlap the 3'' transforming segment, it was concluded that BPV-1 encodes at least 2 genes that can independently transform cultured cells. Mutational analysis of the 5'' transforming segment suggests that the transforming gene of this segment lies within the E6 open reading frame. The 2 transforming segments differ in their biological activity in that the E6-containing fragment can transform C127 mouse cells but not NIH3T3 mouse cells; the 3'' fragment can transform both cell lines.