Metabolites and Lipids Associated with Fetal Swine Anatomy via Desorption Electrospray Ionization-Mass Spectrometry Imaging
Metabolites and Lipids Associated with Fetal Swine Anatomy via Desorption Electrospray Ionization-Mass Spectrometry Imaging
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DOI：
10.1038/s41598-019-43698-2
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发表时间：
2019-05-10
影响因子：
4.6
通讯作者：
Cooks, R. Graham
中科院分区：
文献类型：
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作者：
Leon, Marisol;Ferreira, Christina R.;Cooks, R. Graham
质谱（MS）化学成像已被广泛用于研究动物和人类的疾病，特别是癌症;然而，这项技术已被最低限度地探索研究与胎儿发育相关的复杂化学变化。在这项工作中，我们报告了组织相容的化学成像的小分子解吸电喷雾电离（DESI）- MS的一个完整的猪胎儿在妊娠50天。组织形态不受形态学友好的EST-MS分析的干扰，同时允许检测广泛的小分子。我们观察到脂质的器官依赖性定位，例如，与其他器官相比，脑中的磷脂酰丝氨酸脂质具有很大的多样性，以及发育中的神经系统中的代谢物如N-乙酰基-天冬氨酸和心脏中的N-乙酰基-L-谷氨酰胺。肺中富含的一些脂质，如PC（32：0）和PS（40：6），与以前报道的表面活性剂组成相似。硫酸脂在胎仔肝脏中高度集中，而己糖在该器官中几乎未检测到，但在肺和心脏中含量丰富。小分子的化学信息记录通过EST-MS成像加上传统的解剖学评价是一个强大的生物分析信息的来源，揭示了与胚胎和胎儿发育相关的化学变化，当干扰，导致先天性疾病，如脊柱裂和腭裂。
Chemical imaging by mass spectrometry (MS) has been largely used to study diseases in animals and humans, especially cancer; however, this technology has been minimally explored to study the complex chemical changes associated with fetal development. In this work, we report the histologically-compatible chemical imaging of small molecules by desorption electrospray ionization (DESI) - MS of a complete swine fetus at 50 days of gestation. Tissue morphology was unperturbed by morphologically-friendly DESI-MS analysis while allowing detection of a wide range of small molecules. We observed organ-dependent localization of lipids, e.g. a large diversity of phosphatidylserine lipids in brain compared to other organs, as well as metabolites such as N-acetyl-aspartic acid in the developing nervous system and N-acetyl-L-glutamine in the heart. Some lipids abundant in the lungs, such as PC(32:0) and PS(40:6), were similar to surfactant composition reported previously. Sulfatides were highly concentrated in the fetus liver, while hexoses were barely detected at this organ but were abundant in lung and heart. The chemical information on small molecules recorded via DESI-MS imaging coupled with traditional anatomical evaluation is a powerful source of bioanalytical information which reveals the chemical changes associated with embryonic and fetal development that, when disturbed, causes congenital diseases such as spina bifida and cleft palate.