Ultrastructure of the intima in WHHL and cholesterol-fed rabbit aortas prepared by ultra-rapid freezing and freeze-etching.
Ultrastructure of the intima in WHHL and cholesterol-fed rabbit aortas prepared by ultra-rapid freezing and freeze-etching.
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发表时间：
1989-07
影响因子：
6.5
通讯作者：
J. Frank;A. Fogelman
中科院分区：
文献类型：
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作者：
J. Frank;A. Fogelman
对正常渡边遗传性高脂血症（WHHL）和高胆固醇（10天~ 3个月）家兔主动脉内膜进行了不加化学固定的超快速冷冻和旋转阴影冷冻刻蚀。无细胞区域的细胞外基质非常清晰，由粗丝网、细分支丝、胶原原纤维和大小不一的颗粒组成。正常内膜未见脂质沉积。然而，WHHL内膜的内皮下区域充满胶原原纤维，胶原原纤维围绕并缠绕在大小为23至169 nm的离散脂质囊泡簇之间。WHHL兔内膜中约80%的脂质囊泡在70 ~ 169 nm之间。WHHL内膜的脂质颗粒总是呈簇状，其中许多似乎融合成更大的囊泡。这些聚集体明显与基质细丝相连。在喂胆固醇的家兔的细胞外基质中也观察到类似的脂质颗粒沉积，但与WHHL内膜的颗粒大小分布相反，胆固醇喂入的内膜中超过75%的颗粒直径在23至68 nm之间，51%的颗粒直径在23至45 nm之间。我们得出结论，在WHHL的无细胞区，仅在10天的胆固醇喂养后，脂蛋白衍生的脂质以囊泡团的形式存在于内膜中，包裹在复杂的细胞外基质中。
Intima from aortas of normal Watanabe Heritable Hyperlipidemic (WHHL) and cholesterol-fed (10 days - 3 months) rabbits were examined by ultra-rapid freezing without chemical fixation followed by rotary shadow freeze-etching. The extracellular matrix in areas devoid of cells was seen in extraordinary detail and consisted of a reticulum of thick filaments, finer branching filaments, collagen fibrils, and granules of varying sizes. No lipid deposits were seen in normal intima. However, the subendothelial region of WHHL intima was filled with collagen fibrils surrounding and entwined between clusters of discrete lipid vesicles that ranged in size from 23 to 169 nm. Approximately 80% of the lipid vesicles in the WHHL rabbit intima measured between 70 and 169 nm. The lipid particles in the WHHL intima always appeared in clusters, many of which appeared to be fusing into larger size vesicles. These aggregates were clearly linked to the matrix filaments. A similar deposition of lipid particles was seen in the extracellular matrix of cholesterol-fed rabbits but in contrast to the particle size distribution of the WHHL intima, more than 75% of the particles in the cholesterol-fed intima had a diameter between 23 and 68 nm and 51% were between 23 and 45 nm. We conclude that in cell-free areas of WHHL and after only 10 days of cholesterol feeding, lipoprotein-derived lipid is present in the intima as clusters of vesicles enmeshed in the complex extracellular matrix.