Construction of a high-resolution genetic map and identification of quantitative trait loci for salt tolerance in jute (Corchous spp.)
Construction of a high-resolution genetic map and identification of quantitative trait loci for salt tolerance in jute (Corchous spp.)
复制标题
黄麻（Corchous spp.）高分辨率遗传图谱的构建和耐盐数量性状位点的鉴定
DOI：
10.1186/s12870-019-2004-7
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发表时间：
2019-09
影响因子：
5.3
通讯作者：
Su Jianguang
中科院分区：
文献类型：
--
作者：
Yang Zemao;Yang Youxin;Dai Zhigang;Xie Dongwei;Tang Qing;Cheng Chaohua;Xu Ying;Liu Chan;Deng Canhui;Chen Jiquan;Su Jianguang
背景
黄麻（黄麻属）是仅次于棉花的重要天然纤维作物。盐胁迫严重制约着植物的生长发育。高密度遗传连锁图谱是进行数量性状位点（QTL）定位的基础。通过下一代测序技术，已经在许多作物物种中构建了高密度遗传图谱和与耐盐性相关的QTL定位。然而，这样的研究是罕见的黄麻。迄今为止，在黄麻中仅构建了几张低密度遗传图谱，尚未定位到与耐盐性相关的QTL。
结果
利用黄麻F2群体构建了一张高密度遗传图谱，该图谱包含4839个单核苷酸多态性标记，标记间距为1375.41 cM，标记间平均距离为0.28 cM，标记间的连锁群从LG 2（299个标记，113.66 cM）到LG 7（1542个标记，350.18 cM）。此外，99.57%的相邻标记之间的间隙小于5cM。在4个LGS上共检测到3个显著和13个微效QTL，对表型变异的贡献率为0.58-19.61%。QTL定位的区间长度为1.3 ~ 20.2cM。主效QTL qJST-1在两种盐胁迫条件下检测到，分别解释了11.81%和19.61%的表型变异，并在LG 4上达到峰值19.3 cM。
结论
我们开发了第一个高密度和最完整的黄麻遗传图谱，迄今为止使用基因分型测序方法。首次在黄麻中进行了与耐盐性相关的QTL定位。这些结果为黄麻种子萌发期耐盐性的分子标记辅助选择和转基因育种提供了有用的资源。
Background
Jute (Corchorus spp.) is the most important natural fiber crop after cotton in terms of cultivation area and production. Salt stress greatly restricts plant development and growth. A high-density genetic linkage map is the basis of quantitative trait locus (QTLs) mapping. Several high-density genetic maps and QTLs mapping related to salt tolerance have been developed through next-generation sequencing in many crop species. However, such studies are rare for jute. Only several low-density genetic maps have been constructed and no salt tolerance-related QTL has been mapped in jute to date.
Results
We developed a high-density genetic map with 4839 single nucleotide polymorphism markers spanning 1375.41 cM and an average distance of 0.28 cM between adjacent markers on seven linkage groups (LGs) using an F2 jute population, LGs ranged from LG2 with 299 markers spanning 113.66 cM to LG7 with 1542 markers spanning 350.18 cM. In addition, 99.57% of gaps between adjacent markers were less than 5 cM. Three obvious and 13 minor QTLs involved in salt tolerance were identified on four LGs explaining 0.58–19.61% of the phenotypic variance. The interval length of QTL mapping varied from 1.3 to 20.2 cM. The major QTL, qJST-1, was detected under two salt stress conditions that explained 11.81 and 19.61% of the phenotypic variation, respectively, and peaked at 19.3 cM on LG4.
Conclusions
We developed the first high-density and the most complete genetic map of jute to date using a genotyping-by-sequencing approach. The first QTL mapping related to salt tolerance was also carried out in jute. These results should provide useful resources for marker-assisted selection and transgenic breeding for salt tolerance at the germination stage in jute.
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影响因子：
5.3
作者：
通讯作者：
--
DOI：
--
发表时间：
2011
期刊：
--
影响因子：
--
作者：
Shengchu Wang;C. Basten;Z. Zeng
通讯作者：
Shengchu Wang;C. Basten;Z. Zeng
影响因子：
4.6
作者：
Patil G;Do T;Vuong TD;Valliyodan B;Lee JD;Chaudhary J;Shannon JG;Nguyen HT
通讯作者：
Nguyen HT
影响因子：
64.5
作者：
Zhu, Jian-Kang
通讯作者：
Zhu, Jian-Kang
影响因子：
5.3
作者：
Shi Y;Gao L;Wu Z;Zhang X;Wang M;Zhang C;Zhang F;Zhou Y;Li Z
通讯作者：
Li Z