FePt nanoparticles as a potential X-ray activated chemotherapy agent for HeLa cells.
FePt nanoparticles as a potential X-ray activated chemotherapy agent for HeLa cells.
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FEPT纳米颗粒作为HeLa细胞的潜在X射线激活化学疗法。
DOI：
10.2147/ijn.s88458
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发表时间：
2015
影响因子：
8
通讯作者：
Jiang C
中科院分区：
文献类型：
--
作者：
Zheng Y;Tang Y;Bao Z;Wang H;Ren F;Guo M;Quan H;Jiang C
纳米材料在“个性化”治疗方面具有优势，这是肿瘤治疗的最终目标。为了研究FePt纳米粒子（NPs）在恶性肿瘤诊断和放化疗治疗中的潜在能力，以半胱胺为封端剂，合成了超顺磁性、单分散FePt（~3 nm）合金NPs。通过X射线衍射、透射电子显微镜、物理性能测量系统和傅里叶变换红外光谱对纳米颗粒进行了表征。使用MTT法评估FePt NPs对Vero细胞的细胞毒性，并且还使用MTT法收集由单个FePt NPs和FePt NPs与X射线束组合抑制的肿瘤细胞增殖; HeLa人癌细胞系用作体外模型。使用HeLa细胞验证FePt NPs和X射线的组合效果的进一步确认，之后，通过透射电子显微镜捕获FePt NPs的细胞摄取。结果表明，FePt纳米粒子对HeLa细胞的生长有明显的抑制作用，且呈浓度依赖性，FePt纳米粒子与X射线联合作用对HeLa细胞的抑制作用更强;当浓度<250 μg/mL时，单个纳米粒子对Vero细胞无明显的细胞毒性。同时，在研究结束时，FePt纳米颗粒在MRI/CT分子成像中显示出阴性/阳性对比增强。因此，综合结果表明，FePt纳米颗粒可能作为一个有前途的纳米探针的整合肿瘤诊断和放化疗。
Nanomaterials have an advantage in “personalized” therapy, which is the ultimate goal of tumor treatment. In order to investigate the potential ability of FePt nanoparticles (NPs) in the diagnosis and chemoradiotherapy treatment of malignant tumors, superparamagnetic, monodispersed FePt (~3 nm) alloy NPs were synthesized, using cysteamine as a capping agent. The NPs were characterized by means of X-ray diffraction; transmission electron microscopy, Physical Property Measurement System, and Fourier transform infrared spectroscopy. The cytotoxicity of FePt NPs on Vero cells was assessed using an MTT assay, and tumor cell proliferation inhibited by individual FePt NPs and FePt NPs combined with X-ray beams were also collected using MTT assays; HeLa human cancer cell lines were used as in vitro models. Further confirmation of the combined effect of FePt NPs and X-rays was verified using HeLa cells, after which, the cellular uptake of FePt NPs was captured by transmission electron microscopy. The results indicated that the growth of HeLa cells was significantly inhibited by FePt NPs in a concentration-dependent manner, and the growth was significantly more inhibited by FePt NPs combined with a series of X-ray beam doses; the individual NPs did not display any remarkable cytotoxicity on Vero cells at a concentration <250 μg/mL. Meanwhile, the FePt NPs showed negative/positive contrast enhancement for MRI/CT molecule imaging at the end of the study. Therefore, the combined results implied that FePt NPs might potentially serve as a promising nanoprobe for the integration of tumor diagnosis and chemoradiotherapy.