Very-KIND is a novel nervous system specific guanine nucleotide exchange factor for Ras GTPases
Very-KIND is a novel nervous system specific guanine nucleotide exchange factor for Ras GTPases
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DOI：
10.1016/j.modgep.2005.04.015
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发表时间：
2005-12-01
影响因子：
1.2
通讯作者：
Kerkhoff, E
中科院分区：
文献类型：
--
作者：
Mees, A;Rock, R;Kerkhoff, E
激酶非催化C端半叶结构域（KIND）从催化蛋白激酶折叠进化为信号蛋白的潜在蛋白质相互作用模块。Spir家族肌动蛋白组织者和非受体磷酸酶13型（PTP 13型）在蛋白质的N-末端部分编码KIND结构域。在这里，我们报告的表征和克隆的第三个成员的KIND蛋白家族，我们命名为非常KIND（VKIND），因为它的两个KIND域。与蛋白质家族的其他成员一样，VKIND在N末端具有KIND结构域。第二个KIND结构域位于蛋白质的中心部分。C-末端一半编码Ras样GTP酶的鸟嘌呤核苷酸交换因子基序（RasGEF）和RasGEF N-末端模块（RasGEFN）。在哺乳动物基因组中只有一个VKIND基因，迄今为止，我们只在脊椎动物中发现了该基因。在小鼠胚胎发育过程中，VKIND基因在发育中的神经系统中特异性表达。在成年小鼠中，北方杂交显示仅在大脑中高表达。在卵巢中可检测到低表达。原位杂交结果显示VKIND在小脑颗粒层和浦肯野细胞层的神经元细胞中特异性表达。(c)2005 Elsevier B. V.保留所有权利。
The kinase non-catalytic c-lobe domain (KIND) evolved from the catalytic protein kinase fold into a potential protein interaction module for signalling proteins. Spir family actin organizers and the non-receptor phosphatase type 13 (PTP type 13) encode a KIND domain in the very N-terminal parts of the proteins. Here we report the characterization and cloning of a third member of the KIND protein family, which we have named very-KIND (VKIND) because of its two KIND domains. Like the other members of the protein family, VKIND has a KIND domain at the N-terminus. A second KIND domain is located in the central part of the protein. The C-terminal half encodes a guanine nucleotide exchange factor motif for Ras-like GTPases (RasGEF) and a RasGEF N-terminal module (RasGEFN). There is only one VKIND gene in the mammalian genomes and up to now we have found the gene only in vertebrates. During mouse embryogenesis the VKIND gene was specifically expressed in the developing nervous system. In adult mice Northern hybridizations revealed high expression only in brain. Low expression could be detected in ovary. In situ hybridizations showed a specific expression of VKIND in neuronal cells of the granular and Purkinje cell layers of the cerebellum. (c) 2005 Elsevier B.V. All rights reserved.