Microbiota and SCFA in Lean and Overweight Healthy Subjects
Microbiota and SCFA in Lean and Overweight Healthy Subjects
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DOI：
10.1038/oby.2009.167
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发表时间：
2010-01-01
期刊：
影响因子：
6.9
通讯作者：
Hardt, Philip D.
中科院分区：
文献类型：
--
作者：
Schwiertz, Andreas;Taras, David;Hardt, Philip D.
最近，肥胖被认为与人类微生物区系的组成和短链脂肪酸(SCFA)的产生有关。然而，这些发现依赖于使用相当小和确定的志愿者或模型动物群体进行的实验研究。我们的目的是评估瘦肉型和肥胖型受试者肠道微生物区系和粪便中单链脂肪酸浓度的差异。共有98名受试者自愿参加这项研究。30名志愿者体重指数(Kg/m(2))在瘦范围内，35人超重，33人肥胖。用实时荧光定量聚合酶链式反应分析粪便微生物区系。用本文所用的引物，我们能够覆盖用通用引物可检测到的总微生物区系的82.3%(四分位数范围为68.3-91.4%)。此外，还对SCFA的浓度进行了评价。肥胖组SCFA总量高于瘦身组(P=0.024)。在超重(P=0.019)和肥胖者(P=0.028)中，个体SCFA的比例倾向于丙酸。瘦肉型和肥胖型人群粪便中最丰富的细菌群为拟杆菌门和拟杆菌门。在超重(P=0.001)和肥胖者(P=0.005)中，细菌与类杆菌的比值向类杆菌倾斜。我们的结果与以前的报道一致，表明SCFA代谢在肥胖中可能起着相当大的作用。然而，我们的结果与之前关于各种细菌群对肥胖发展的贡献的报告相矛盾，这一问题仍然存在争议。
Obesity has recently been linked to the composition of human microbiota and the production of short chain fatty acids (SCFAs). However, these findings rely on experimental studies carried out using rather small and defined groups of volunteers or model animals. Our aim was to evaluate differences within the human intestinal microbiota and fecal SCFA concentration of lean and obese subjects. A total of 98 subjects volunteered to take part in this study. The BMI in kg/m(2) of 30 volunteers was within the lean range, 35 were overweight and 33 were obese. The fecal microbiota was characterized by real-time PCR analyses. With the primers used herein we were able to cover 82.3% (interquartile range of 68.3-91.4%) of the total microbiota detectable with a universal primer. In addition, the concentration of SCFA was evaluated. The total amount of SCFA was higher in the obese subject group (P = 0.024) than in the lean subject group. The proportion of individual SCFA changed in favor of propionate in overweight (P = 0.019) and obese subjects (P = 0.028). The most abundant bacterial groups in faeces of lean and obese subjects belonged to the phyla Firmicutes and Bacteroidetes. The ratio of Firmicutes to Bacteroidetes changed in favor of the Bacteroidetes in overweight (P = 0.001) and obese subjects (P = 0.005). Our results are in line with previous reports suggesting that SCFA metabolism might play a considerable role in obesity. However, our results contradict previous reports with regard to the contribution of various bacterial groups to the development of obesity and this issue remains controversial.