SELECTIVE DEPLETION OF RAT NEUTROPHILS BY INVIVO ADMINISTRATION OF A MONOCLONAL-ANTIBODY
SELECTIVE DEPLETION OF RAT NEUTROPHILS BY INVIVO ADMINISTRATION OF A MONOCLONAL-ANTIBODY
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DOI：
10.1002/jlb.46.2.96
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发表时间：
1989-08-01
影响因子：
5.5
通讯作者：
SENDO, F
中科院分区：
文献类型：
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作者：
SEKIYA, S;GOTOH, S;SENDO, F
用WKA/Hok大鼠腹腔中性粒细胞致敏的BALB/c小鼠脾细胞与小鼠骨髓瘤细胞（P3-X63.Ag8.653）杂交，制备了一种选择性清除大鼠中性粒细胞的单克隆抗体（RP-3）。RP-3与大鼠中性粒细胞反应，但不与淋巴细胞、巨噬细胞、自然杀伤细胞、嗜碱性粒细胞、嗜酸性粒细胞或各种器官的组织反应。有丝分裂反应伴刀豆球蛋白A（ConA），植物血凝素（PHA），脂多糖（LPS）或大鼠给予RP-3是没有显着不同的正常大鼠。将RP-3注射到保持在无特定病原体（SPF）或清洁条件下的大鼠的腹腔中，可诱导循环中性粒细胞选择性消耗至100/mm 3（0.5% WBC）以下。单核细胞、淋巴细胞和血小板的数量未发生变化。给予2 ml RP-3使血液中性粒细胞减少至100/mm 3以下约24 h，给予1 ml引起耗竭约12 h。
A monoclonal antibody (RP-3) that depletes rat neutrophils selectively in vivo was developed by hybridization of mouse myeloma cells (P3-X63.Ag8.653) and spleen cells of BALB/c mice sensitized with peritoneal neutrophils of WKA/Hok rats. RP-3 reacted with rat neutrophils but not with lymphocytes, macrophages, natural killer cells, basophils, eosinophils, or tissues of various organs. The mitogenic responsiveness to concanavalin A (ConA), phytohemagglutinin (PHA), and lipopolysaccharide (LPS) or rats given RP-3 was not significantly different from that of normal rats. Administration of RP-3 into the peritoneal cavity of rats that had been kept under specific pathogen-free (SPF) or clean conditions induced selective depletion of circulating neutrophils to under 100/mm3 (0.5% WBC). The numbers of monocytes, lymphocytes, and platelets were not changed. Administration of 2 ml of RP-3 reduced blood neutrophils to under 100/mm3 for approximately 24 h, and administration of 1 ml caused depletion for approximately 12 h.