THE NEURON-RESTRICTIVE SILENCER FACTOR (NRSF) - A COORDINATE REPRESSOR OF MULTIPLE NEURON-SPECIFIC GENES
THE NEURON-RESTRICTIVE SILENCER FACTOR (NRSF) - A COORDINATE REPRESSOR OF MULTIPLE NEURON-SPECIFIC GENES
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DOI：
10.1126/science.7871435
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发表时间：
1995-03-03
期刊：
影响因子：
56.9
通讯作者：
ANDERSON, DJ
中科院分区：
文献类型：
--
作者：
SCHOENHERR, CJ;ANDERSON, DJ
神经元限制性沉默因子 (NRSF) 结合 DNA 序列元件，称为神经元限制性沉默元件 (NRSE)，抑制非神经元细胞中的神经元基因转录。共有 NRSE 已在 18 个神经元特异性基因中被鉴定，编码 NRSF 功能片段的互补 DNA 克隆被分离出来，并发现编码含有八个非规范锌指的新蛋白质。在多个发育阶段的大多数非神经元组织中检测到 NRSF mRNA 的表达。在神经系统中，在未分化的神经元祖细胞中检测到 NRSF mRNA，但在分化的神经元中未检测到。NRSF 代表了脊椎动物沉默蛋白的第一个例子，该蛋白可能调节大量细胞类型特异性基因，因此可能充当神经发生的主要负调节因子。
The neuron-restrictive silencer factor (NRSF) binds a DNA sequence element, called the neuron-restrictive silencer element (NRSE), that represses neuronal gene transcription in nonneuronal cells. Consensus NRSEs have been identified in 18 neuron-specific genes, Complementary DNA clones encoding a functional fragment of NRSF were isolated and found to encode a novel protein containing eight noncanonical zinc fingers. Expression of NRSF mRNA was detected in most nonneuronal tissues at several developmental stages, In the nervous system, NRSF mRNA was detected in undifferentiated neuronal progenitors, but not in differentiated neurons, NRSF represents the first example of a vertebrate silencer protein that potentially regulates a large battery of cell type-specific genes, and therefore may function as a master negative regulator of neurogenesis.